FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688910
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACCGATGGCCAGGGAGG38920.564950428938468No Hit
GACTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGCGTAATGTTCGG30240.4389542901104644No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25810.3746498091187528No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAACATTACGCCACTCAGGCTGTCATCCCATG20610.2991682512955248No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19580.28421709657284694No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19520.2833461555210405No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18090.2625887271196528No Hit
GACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAACATTACGCCACTCAGG17070.24778272923894265No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTC15590.22629951662771625No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15490.2248479482080388No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15170.2202029292650709No Hit
CTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGCGTAATGTTCGGCG15000.2177352629516192No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14480.21018710716929645No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13720.19915518717974773No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13310.1932037566590701No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13300.19305859981710238No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13190.19146187455545718No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12100.17563977878097284No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12040.17476883772916638No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11600.16838193668258553No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC11350.16475301563339187No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11290.1638820745815854No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10950.15894674195468203No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10920.1585112714287788No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10850.15749517353500458No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10750.1560436051153271No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10700.15531782090548837No Hit
CAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACCGATGGCCAGGGA10700.15531782090548837No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10530.15285015459203669No Hit
GTCTCTGGTGATTCCATCGATGGTTACTACTGGAGCTGGATCCGGCAGCC10460.15183405669826247No Hit
CCCCTGAGGGCCGCTGATTAGAATTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGAATA10420.1512534293303915No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10210.14820513564906881No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9940.14428590091593968No Hit
CAATAATACACGGCCGTGTCCGCAGTGGTCACAGAAGTCAACTTCAGGGAGATCTGGTTCTTGGACATGTCTA9710.14094729355068153No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9670.14036666618281055No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9660.14022150934084276No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9380.13615711776574588No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9290.13485070618803618No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG9260.13441523566213295No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9060.131512098822778No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9010.1307863146129393No Hit
ATAATACACGGCCGTGTCCGCAGTGGTCACAGAAGTCAACTTCAGGGAGA8980.13035084408703604No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8850.12846380514145533No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT8810.12788317777358435No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8810.12788317777358435No Hit
GTCCTGGACTTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGGC8640.1254155114601327No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8530.12381878619848748No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT8530.12381878619848748No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8480.12309300198864873No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8470.12294784514668099No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8360.12135111988503579No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCGGTGGGCTCCGGT8330.12091564935913254No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8260.11989955146535833No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATTATGTATTCTGGTACCAGCAACTCCCAGGAACGGCCCC8070.11714157146797113No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT8060.1169964146260034No Hit
ACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAACATTACGCCACTCAGGC8040.11670610094206792No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7910.11481906199648721No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7870.11423843462861623No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7860.11409327778664846No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7710.11191592515713228No Hit
GCATGGGATGACAGCCTGAGTGGCGTAATGTTCGGCGGAGGGACCCGGCTGACCGTCCTAGGTCAGCCCAAGG7710.11191592515713228No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7690.1116256114731968No Hit
GTATTATTGTGCGAAAGATCGATACGGATTGCGGTGGCCTGACTTTGACTATTGGGGCCGGGGGACCCTGGTCAC7670.1113352977892613No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG7590.11017404305351934No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7560.1097385725276161No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG7550.10959341568564833No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7480.10857731779187411No Hit
GAATTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCA7470.10843216094990638No Hit
CACCTGCACTGTCTCTGGTGATTCCATCGATGGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGG7390.10727090621416441No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC7370.1069805925302289No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7320.10625480832039018No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7160.10393229884890624No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7110.10320651463906752No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7090.10291620095513201No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7030.10204525990332554No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTC6910.10030337779971259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.002373834468.86252639
GTAGTTT652.0533322E-550.12792270
AGGGGGG2500.049.52638670
CGTATGC1700.046.7272941
TCGTATG1700.046.65526640
GCCAGAG13100.045.26819270
GGTATCA5050.043.9151951
CACGAGA2000.042.62983721
ATGCCGT1900.041.97524644
ACGAGAC2150.041.24488422
GCCGTCT1950.041.01701446
GTGGTAT1450.040.001271
CCCTAAG2050.039.99033728
TGCTTGA2150.039.39923555
TCTTATC350.001426140138.9758532
CCTATCC350.001426140138.9758533
CCGAAAT459.4953306E-538.67929547
GTATCAA13100.038.28591
ACTTTCC451.0279186E-438.16187737
CCGTCTT2100.038.1267347
Produced by FastQC (version 0.11.5)