FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688910
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGCGTAATGTTCGG30440.4418574269498193No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23710.3441668723055261No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC21950.3186192681192028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19990.29016852709352453No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18650.2707175102698466No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18020.2615726292258786No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT16980.24647631766123299No Hit
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACCGATGGCCAGGGAGG16730.2428473966120393No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAACATTACGCCACTCAGGCTGTCATCCCATG16040.23283157451626482No Hit
CTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGCGTAATGTTCGGCG14560.21134836190503842No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14060.20409051980665108No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14000.20321957875484462No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13890.20162285349319942No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13600.19741330507613478No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13260.1924779724492314No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13200.1916070313974249No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTC13170.19117156087152168No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13010.18884905140003774No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11990.17404305351932764No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11890.17259148509965017No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11750.1705592893121017No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11740.17041413247013398No Hit
GACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAACATTACGCCACTCAGG11670.16939803457635974No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11470.16649489773700482No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11450.16620458405306934No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11060.16054346721632726No Hit
GTCTCTGGTGATTCCATCGATGGTTACTACTGGAGCTGGATCCGGCAGCC11030.160107996690424No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10380.1506728019625205No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10330.1499470177526818No Hit
CCCCTGAGGGCCGCTGATTAGAATTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGAATA9880.14341495986413322No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9700.14080213670871375No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9680.14051182302477827No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATTATGTATTCTGGTACCAGCAACTCCCAGGAACGGCCCC9670.14036666618281055No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9380.13615711776574588No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9190.1333991377683587No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC9160.13296366724245548No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG9150.13281851040048773No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9090.13194756934868127No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCGGTGGGCTCCGGT8880.1288992756673586No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8760.1271573935637456No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8690.1261412956699714No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8610.12498004093422943No Hit
CAATAATACACGGCCGTGTCCGCAGTGGTCACAGAAGTCAACTTCAGGGAGATCTGGTTCTTGGACATGTCTA8550.12410909988242295No Hit
ATAATACACGGCCGTGTCCGCAGTGGTCACAGAAGTCAACTTCAGGGAGA8370.12149627672700353No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC8330.12091564935913254No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8230.11946408093945508No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT8190.1188834535715841No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8160.11844798304568085No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT8150.11830282620371312No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8100.11757704199387438No Hit
CACCTGCACTGTCTCTGGTGATTCCATCGATGGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGG8080.1172867283099389No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8060.1169964146260034No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7900.11467390515451947No Hit
GTATTATTGTGCGAAAGATCGATACGGATTGCGGTGGCCTGACTTTGACTATTGGGGCCGGGGGACCCTGGTCAC7820.11351265041877748No Hit
GTCCTGGACTTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGGC7750.11249655252500326No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7720.11206108199910002No Hit
GCATGGGATGACAGCCTGAGTGGCGTAATGTTCGGCGGAGGGACCCGGCTGACCGTCCTAGGTCAGCCCAAGG7670.1113352977892613No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7650.1110449841053258No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7620.11060951357942256No Hit
GAATTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCA7420.10770637674006765No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7160.10393229884890624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)