FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887458
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT47300.5329829693348869No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36420.41038561824897635No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC32230.36317211631423685No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31720.3574253654820848No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACGAAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG31100.35043911937240974No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28520.3213673210450523No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24790.27933716299813627No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24410.2750552702212386No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT23510.2649139452233233No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22890.2579276991136482No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22330.2516175413371675No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC21800.24564542772728398No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21390.2410254907837892No Hit
GGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC20710.23336315634091978No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG19560.22040479662136123No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCTTCGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG19460.21927798273270396No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGAGAGGTAGC19310.2175877618997181No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA19190.21623558523332936No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18780.21161564828983456No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18720.2109395599566402No Hit
GTACTTGATGAAGCTTAACAATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG18500.20846056940159421No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18260.20575621606881678No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT18190.20496744634675668No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18080.2037279510692337No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17920.20192504884738205No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17040.19200908662719815No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16730.1885159635723606No Hit
CTATTACTGTCAGACCTGGGGCACTGGCTTCGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCC16240.18299457551794002No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15900.17916340829650532No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCGTGGCATC15810.17814927579671375No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15650.17634637357486213No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15500.17465615274187624No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA15370.1731912946866218No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCGTGGCATCAG15230.1716137552425016No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15170.17093766690930726No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT14660.1651909160771552No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG14280.16090902330025758No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14120.15910612107840597No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC13910.1567398119122257No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGAGAGGTAGCGCTCAGCCCCAGAGCTGGAGC13870.1562890863567628No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT13530.1524579191353281No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13520.15234523774646236No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13430.15133110524667084No Hit
GCTCTGGGGCTGAGCGCTACCTCTCCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC13230.1490774774693563No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13120.1478379821918333No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12860.14490826608132443No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12520.14107709885988973No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCTTCG11830.13330208302815455No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG11710.13194990636176585No Hit
TCCTCAGACTGGAGACTGGAGATGGAGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC11700.13183722497290012No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT11690.1317245435840344No Hit
CCTTGGTCCCTCCGCCGAATACCACGAAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA11590.13059772969537714No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGC11550.13014700413991423No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11420.12868214608465978No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACGAAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC11370.12811873914033114No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC11220.12642851830734525No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11030.12428757191889644No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10830.1220339441415819No Hit
GTATATTACTGTGCGATCCCTGCCCGTAATTCCGGTCCGCTTAACTACTGGGGCCAGGGAACCCTGGTCACCGT10780.12147053719725329No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10700.12056908608632746No Hit
CTCCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCTTCGTGGTATT10690.12045640469746173No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA10650.12000567914199883No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC10610.11955495358653592No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG10570.11910422803107301No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC10500.11831545830901294No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10420.11741400719808713No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10190.11482233525417541No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10160.11448429108757822No Hit
ATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCACGCGATGGC10140.11425892830984677No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9640.10862485886656045No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCT9500.10704731942244028No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCTCCATCTCCAGTCTCCAGTCTG9330.10513173581172292No Hit
GCTTAACAATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT9300.10479369164512575No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9280.1045683288673943No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9270.10445564747852856No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9170.1033288335898713No Hit
TTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCACGCGATGGCGTAGCTGCTGTGC9130.10287810803440839No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9100.1025400638678112No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGC9090.10242738247894549No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9090.10242738247894549No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC9080.10231470109007974No Hit
CTACCTCTCCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACT8990.10130056859028821No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8950.10084984303482532No Hit
CCCTTGGTGTGGCTGCCATCATTGTTAAGCTTCATCAAGTACCGAGGGCC8910.1003991174793624No Hit
GTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC8910.1003991174793624No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC8880.10006107331276522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6700.089.9556870
CTCTTAT1600.048.4731141
CTTATAC8500.048.398731
TATACAC8850.046.855123
GGTATCA8300.046.7210731
TTATACA8850.046.4741862
CGTATGC7550.046.21901741
TCGTATG7400.046.11028740
ATGCCGT7550.045.63845444
TATCTCG7350.045.56137536
GTATCGC606.162736E-945.51199735
GTATGCC7950.045.29406442
TCTCGTA7200.045.2807238
ACCGATC8850.045.08252327
TCAGTAT8800.044.82095332
ATCTCCG9250.044.46699510
TGCTTGA7350.044.2449755
ATACACA9400.044.1135944
CCGATCA9150.044.01870728
CCCACGA9150.043.85745619
Produced by FastQC (version 0.11.5)