FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887458
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88740.9999346447944578No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38950.4388940096320051No Hit
GGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC33800.38086309436615595No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29170.3286916113213245No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC28490.32102927687845506No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26810.30209880354901303No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACGAAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG24570.2768581724430903No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT23000.2591671943911712No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22750.256350159669528No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22620.2548853016142736No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22460.25308239939242194No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21810.2457581091161497No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG19700.22198233606548143No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19480.21950334551043543No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCTTCGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG19030.2144326830114777No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC18800.21184101106756603No Hit
GTACTTGATGAAGCTTAACAATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG18660.21026347162344583No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18400.20733375551293692No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18210.20519280912448817No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18030.20316454412490506No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG17860.20124896051418772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17630.198657288570276No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC17350.19550220968203566No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16460.18547356607298598No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGAGAGGTAGC16430.18513552190638882No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16150.18198044301814845No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15430.17386738301981616No Hit
CTATTACTGTCAGACCTGGGGCACTGGCTTCGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCC15350.17296593190889034No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15140.17059962274271007No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT15020.16924744607632136No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA14750.16620504857694673No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCGTGGCATC14710.16575432302148382No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14650.16507823468828947No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT14540.1638387394107665No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCGTGGCATCAG14440.1627119255221092No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14400.1622611999666463No Hit
GCTCTGGGGCTGAGCGCTACCTCTCCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC14250.16057097913366042No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGC13590.15313400746852246No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13520.15234523774646236No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13440.15144378663553654No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13340.15031697274687927No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12670.1427673196928756No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12640.14242927552627843No Hit
GTATATTACTGTGCGATCCCTGCCCGTAATTCCGGTCCGCTTAACTACTGGGGCCAGGGAACCCTGGTCACCGT12630.1423165941374127No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT12470.14051369191556107No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12420.13995028497123244No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG12410.13983760358236672No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12280.13837274552711226No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12010.13533034802773766No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11790.13285135747269167No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGAGAGGTAGCGCTCAGCCCCAGAGCTGGAGC11610.13082309247310858No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCTTCG11310.1274426508071368No Hit
CTCCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCTTCGTGGTATT11120.125301704418688No Hit
TCCTCAGACTGGAGACTGGAGATGGAGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC11080.12485097886322508No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11000.12394952775229925No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC10930.12316075803023918No Hit
CTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT10420.11741400719808713No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10350.11662523747602703No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10320.11628719330942984No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCT10280.11583646775396694No Hit
CCTTGGTCCCTCCGCCGAATACCACGAAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA10190.11482233525417541No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC10140.11425892830984677No Hit
GTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG9890.11144189358820362No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9830.11076580525500926No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9710.10941362858862053No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9690.10918826581088907No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9640.10862485886656045No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC9640.10862485886656045No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCTCCATCTCCAGTCTCCAGTCTG9610.10828681469996326No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9530.10738536358903744No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACGAAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC9510.107160000811306No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC9320.10501905442285721No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9050.10197665692348258No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8930.10062448025709386No Hit
GCTTAACAATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT8880.10006107331276522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT400.007111937752.1933470
TGTACGT200.008120554550.4759142
GGTATCA7950.046.160721
GTATCAA21000.039.759931
TATCAAC24100.034.4883842
ATCAACG25100.033.2465363
TCAACGC25150.033.180444
CAACGCA25750.032.4073035
AACGCAG25850.032.153586
TCTGGAG6500.032.1189870
TGGTATC2250.031.4072362
CGCAGAG26900.030.9002558
GCAGAGT27350.030.5148859
AGAGTAC27400.030.459211
CAGAGTA27700.030.1293210
CGGTGAG450.005044006730.12381630
GATCGTT450.005219944329.91333611
ATGTTCG450.005219944329.9133369
GACAGGT953.965306E-728.3517321
AGTATTG500.00856966627.02555727
Produced by FastQC (version 0.11.5)