FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969310
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34950.36056576327490686No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33420.3447813393032157No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23840.24594814868308384No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22030.22727507195840338No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19800.2042690161042391No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18880.19477772848727445No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17950.1851832747005602No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17520.18074712940132673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17190.17734264579958942No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17090.17631098410209325No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT17000.1753824885743467No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16490.1701210139171163No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16280.16795452435237437No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15390.15877273524465857No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14720.15186060187143433No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTT14600.15062260783443893No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14590.1505194416646893No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14470.1492814476276939No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14170.14618646253520545No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14110.14556746551670777No Hit
GTGCAGCCACAGTTCGATTCACATCCACTTTGGTCCCAGGGCCGAAACTG13680.1411313202174743No Hit
GATCTATGCTGCATCCATTTTACAAAATGGGGTCTCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCT13190.1360761778997431No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13190.1360761778997431No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12960.13370335599550195No Hit
GTACTGTGACTCTGTTGACAGTAGTAAGACGCAAAATCTTCAGGTAGCAGACGGCTGATGGTGAGAGAGAAAT12920.1332906913165035No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12830.13236219578875694No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12540.1293703768660181No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12030.1241089022087877No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11870.12245824349279384No Hit
GATCAGGAGTTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTCAAAT11660.12029175392805191No Hit
GTAATAGACAGCCGTGTCCTCAGATCTCAGGCCGCTCAGCTCCATGTAGG11650.1201885877583023No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGACAGATATTTGAATTGGTATCAGCAGAAA11480.11843476287255884No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTCACATCCACTTTGGTCCCAGGGCCGAAACTGAACAAGGTA11290.11647460564731614No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11210.1156492762893192No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11080.1143081160825742No Hit
GTCTATTACTGTGCGAGATATGATGGCTGGTACGGAATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC11000.11348278672457728No Hit
CTCCTGATCTATGCTGCATCCATTTTACAAAATGGGGTCTCGTCAAGGTT10960.11307012204557881No Hit
ACCTTGTTCAGTTTCGGCCCTGGGACCAAAGTGGATGTGAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTC10890.1123479588573315No Hit
GCATAGATCAGGAGTTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT10700.11038780163208879No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10700.11038780163208879No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10560.10894347525559418No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10530.10863397674634534No Hit
GAACAAGGTACTGTGACTCTGTTGACAGTAGTAAGACGCAAAATCTTCAG10420.10749914887909957No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10370.10698331803035148No Hit
GTCACAGTACCTTGTTCAGTTTCGGCCCTGGGACCAAAGTGGATGTGAAT10190.10512632697485841No Hit
GTAGTAAGACGCAAAATCTTCAGGTAGCAGACGGCTGATGGTGAGAGAGAAATCTGTCCCAGATCCACTGCCA10010.10326933591936532No Hit
AGTCAGAACATTGACAGATATTTGAATTGGTATCAGCAGAAACCAGGGAAAGTCCCTAAACTCCTGATCTATG9970.10285667124036686No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9940.102547172731118No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9870.10182500954287071No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9760.10069018167562493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3450.047.03704541
TCGTATG3450.046.943440
ATCTCGT3300.042.60000237
GAGACTA3850.042.3466724
GTATGCC3950.042.02990342
TATACAC4000.041.5627373
TTATACA4200.041.199212
CCGTCTT4100.040.83579347
ACTAGGC4000.040.81135627
GACTAGG4100.040.6244526
AGACTAG4050.040.2679525
ATCTCCG4050.040.21186410
CCCACGA4000.039.880719
CTAGGCA4200.039.70028728
CTTATAC4200.039.606031
CCTGGTA4850.039.53083470
TAGGCAT4250.039.25759529
CGAGACT4250.038.34921623
TCTCCGA4350.038.21860511
ATGCCGT4400.037.86279344
Produced by FastQC (version 0.11.5)