FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969310
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34300.3538599622411819No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33250.3430275144174722No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32540.3357027163652495No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24350.25120962334031427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20470.21118114947746336No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19120.19725371656126525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17310.1785806398365848No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16670.1719780049726094No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16070.16578803478763243No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15840.16341521288339128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15420.15908223375390743No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15040.15516191930342202No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14920.15392392526642665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14500.1495909461369428No Hit
GATCTATGCTGCATCCATTTTACAAAATGGGGTCTCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCT14470.1492814476276939No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14190.1463927948747047No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTT14190.1463927948747047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.14546429934695815No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13690.1412344863872239No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13210.13628251023924234No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13100.1351476823719966No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13060.13473501769299812No Hit
GTACTGTGACTCTGTTGACAGTAGTAAGACGCAAAATCTTCAGGTAGCAGACGGCTGATGGTGAGAGAGAAAT12950.13360018982575234No Hit
GTGCAGCCACAGTTCGATTCACATCCACTTTGGTCCCAGGGCCGAAACTG12400.12792605048952346No Hit
ACCTTGTTCAGTTTCGGCCCTGGGACCAAAGTGGATGTGAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTC12390.12782288431977387No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12330.12720388730127616No Hit
CTCCTGATCTATGCTGCATCCATTTTACAAAATGGGGTCTCGTCAAGGTT12040.12421206837853732No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11920.12297407434154192No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11610.11977592307930383No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11560.11926009223055575No Hit
GTCTATTACTGTGCGAGATATGATGGCTGGTACGGAATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC11270.1162682733078169No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGACAGATATTTGAATTGGTATCAGCAGAAA10970.11317328821532843No Hit
GATCAGGAGTTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTCAAAT10720.11059413397158804No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10700.11038780163208879No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10670.11007830312283996No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10590.10925297376484303No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10510.10842764440684612No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10380.1070864842001011No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTCACATCCACTTTGGTCCCAGGGCCGAAACTGAACAAGGTA10340.10667381952110264No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10280.10605482250260494No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9930.10244400656136839No Hit
GTAGTAAGACGCAAAATCTTCAGGTAGCAGACGGCTGATGGTGAGAGAGAAATCTGTCCCAGATCCACTGCCA9920.10234084039161878No Hit
GAACAAGGTACTGTGACTCTGTTGACAGTAGTAAGACGCAAAATCTTCAG9800.1011028463546234No Hit
GCATAGATCAGGAGTTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT9700.10007118465712724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)