FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014822
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCAGGTGCAGATACA50680.4993979239709033No Hit
GTATATTACTGTGCGAAACCCCTGGTGGGGTTTATTCAACCAACTAGTGG37270.3672565238041745No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30580.30133363289325615No Hit
GTATGGGACCTCACTTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28870.2844833872344116No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26750.2635930241953761No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTCTT25340.24969896198545166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24830.24467345012228747No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21480.2116627349426796No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20990.2068343019761101No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20620.20318834238910863No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT19860.19569934431851102No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19570.19284170031788828No Hit
GTCCCATACTGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTC19530.1924475425246989No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19450.19165922693832022No Hit
CACTTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC18860.18584539948877735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17960.17697684914201703No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT17640.17382358679650226No Hit
GTTTTACTGTCAGCAGTATGGGACCTCACTTGGGACGTTCGGCCAAGGGA17610.17352796845161023No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17310.17057178500269013No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16690.16446233920825523No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16490.16249155024230852No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16210.15973244568998307No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15960.15726895948254965No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15880.15648064389617097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15850.15618502555127894No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15810.1557908677580896No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15560.15332738155065617No Hit
GTATCTGCACCTGAACAGCCTGAGAGGCGAGGACACGGCCGTATATTACT15550.15322884210235882No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15450.15224344761938546No Hit
GTGTGAGGGGCTGTTAGTGGAGTCTGGGGGAGGCTTGGTTCAGCCTGGGGGGTCCCTAAGACTCTCATGTCTAGC15360.15135659258470943No Hit
AACACATACTACGCAGAGTCCGTGAAGGGCCGACTCACCATCTCCAGAGACAATTCCGACAACACACTGTATCTG15340.15115951368811475No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15330.15106097423981743No Hit
GTGTTAGTACCAATTCCTTAGCCTGGTACCAGCAAAAACCTGGCCAGTCT15280.15056827699833075No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGTGCCATCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAC14960.14741501465281595No Hit
TCCCAAGTGAGGTCCCATACTGCTGACAGTAAAACACTGCAAAATCTTCA14910.14692231741132927No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14900.14682377796303192No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14870.14652815961813992No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14610.14396613396240918No Hit
CCCTAAGACTCTCATGTCTAGCCTCTGGATTCCCCTTTAGCAACTATGCC14240.14032017437540772No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14190.13982747713392102No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.138940622099245No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14020.1381523065128663No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13940.1373639909264876No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGACACGGTGACCATTGTCCCTTGG13570.13371803133948615No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13540.13342241299459412No Hit
GTATTAGTGGAGGTGGCGGTAACACATACTACGCAGAGTCCGTGAAGGGCCGACTCACCATCTCCAGAGACAATT13430.1323384790633234No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13330.13135308458035005No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13230.1303676900973767No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13180.12987499285589No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13070.12879105892461928No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGTGCCATCCTCTCCTGC13050.1285939800280246No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTCTTCTTGT13050.1285939800280246No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13030.12839690113142993No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12860.12672173051037522No Hit
GTATGTGTTACCGCCACCTCCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT12630.12445532319953648No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTCT12460.12278015257848175No Hit
CAGTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCAGGTGCAGATA12350.12169621864721103No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12010.11834587740510158No Hit
GTCTTTGTCTCCAGGGGAAGGTGCCATCCTCTCCTGCAGGGCCAGTCAGA11720.11548823340447882No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGACACGGTGACCATTGTCCCTTGG11720.11548823340447882No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11680.11509407561128948No Hit
CAGTAAAACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11570.11401014168001876No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11560.11391160223172142No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11490.11322182609364007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11480.11312328664534273No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11420.11253204995555872No Hit
ATATTACTGTGCGAAACCCCTGGTGGGGTTTATTCAACCAACTAGTGGTT11250.11085687933450397No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11120.10957586650663859No Hit
GAGTTTGGGCTGAGCTGGCTTCTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGGGCTGTTAGTGGAGT11100.10937878761004394No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11070.10908316926515192No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG11020.10859047202366524No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10980.10819631423047589No Hit
GTGCCATCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTACCAATTCCTTA10910.10750653809239452No Hit
GTCCGTGAAGGGCCGACTCACCATCTCCAGAGACAATTCCGACAACACAC10760.10602844636793449No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAACCACTAGTTGGTTGAATAAAC10550.10395911795369041No Hit
GGACAATGGTCACCGTGTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC10540.10386057850539307No Hit
GACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTGTTTTACTGTCAGCAGTATGGGACCTCA10450.10297372347071704No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10410.10257956567752768No Hit
CCTCATCTACGGTGCATTTAGAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10400.10248102622923035No Hit
GGGCTGGAGTGGGTCTCAAGTATTAGTGGAGGTGGCGGTAACACATACTA10360.102086868436041No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGCATAGTTGC10240.10090439505647296No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10180.10031315836668893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)