FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014822
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCAGGTGCAGATACA46290.4561391061683724No Hit
GTATATTACTGTGCGAAACCCCTGGTGGGGTTTATTCAACCAACTAGTGG35350.3483369497310858No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30540.3009394751000668No Hit
GTATGGGACCTCACTTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27180.2678302204721616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24290.23935231991423128No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTCTT23600.23255309798171503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20940.2063416047346234No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20370.2007248561816752No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19320.19037821411045483No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19240.18958989852407615No Hit
GTCCCATACTGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTC19090.1881118067996161No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18900.1862395572819667No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT18890.18614101783366935No Hit
CACTTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC18160.17894763810796377No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17550.1729367317618262No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17480.17224695562374487No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17190.16938931162312207No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17020.16771414100206736No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT16790.16544773369122862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16780.16534919424293126No Hit
GTATCTGCACCTGAACAGCCTGAGAGGCGAGGACACGGCCGTATATTACT16520.16278716858720052No Hit
GTTTTACTGTCAGCAGTATGGGACCTCACTTGGGACGTTCGGCCAAGGGA16130.15894413010360436No Hit
GTGTTAGTACCAATTCCTTAGCCTGGTACCAGCAAAAACCTGGCCAGTCT15970.157367498930847No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15560.15332738155065617No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15340.15115951368811475No Hit
GTGTGAGGGGCTGTTAGTGGAGTCTGGGGGAGGCTTGGTTCAGCCTGGGGGGTCCCTAAGACTCTCATGTCTAGC15210.14987850086024937No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15120.14899164582557334No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15060.14840040913578934No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15020.14800625134259998No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14810.1459369229283559No Hit
TCCCAAGTGAGGTCCCATACTGCTGACAGTAAAACACTGCAAAATCTTCA14770.14554276513516656No Hit
AACACATACTACGCAGAGTCCGTGAAGGGCCGACTCACCATCTCCAGAGACAATTCCGACAACACACTGTATCTG13810.13608297809862221No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13660.13460488637416218No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGTGCCATCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAC13490.13292971575310744No Hit
CCCTAAGACTCTCATGTCTAGCCTCTGGATTCCCCTTTAGCAACTATGCC13390.13194432127013406No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGTGCCATCCTCTCCTGC13330.13135308458035005No Hit
GTATTAGTGGAGGTGGCGGTAACACATACTACGCAGAGTCCGTGAAGGGCCGACTCACCATCTCCAGAGACAATT13250.13056476899397135No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13230.1303676900973767No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12950.12760858554505125No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12830.12642611216548322No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12770.1258348754756992No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGACACGGTGACCATTGTCCCTTGG12630.12445532319953648No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTCT12430.12248453423358972No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTCTTCTTGT12400.12218891588869772No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12320.12140060030231903No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12280.12100644250912969No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12250.12071082416423766No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12220.12041520581934566No Hit
GTATGTGTTACCGCCACCTCCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT12220.12041520581934566No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12120.11942981133637229No Hit
GAGTTTGGGCTGAGCTGGCTTCTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGGGCTGTTAGTGGAGT12110.11933127188807496No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12040.1186414957499936No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11920.11745902237042555No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11890.11716340402553355No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11820.11647362788745218No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGCATAGTTGC11800.11627654899085751No Hit
GTCTTTGTCTCCAGGGGAAGGTGCCATCCTCTCCTGCAGGGCCAGTCAGA11700.11529115450788414No Hit
CAGTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCAGGTGCAGATA11580.1141086811283161No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11570.11401014168001876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11550.1138130627834241No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11480.11312328664534273No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11450.11282766830045073No Hit
ATATTACTGTGCGAAACCCCTGGTGGGGTTTATTCAACCAACTAGTGGTT11370.11203935271407203No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11320.11154665547258534No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11200.11036418209301731No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGACACGGTGACCATTGTCCCTTGG10980.10819631423047589No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10950.10790069588558386No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10900.1074079986440972No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10730.10573282802304246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10690.1053386702298531No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG10610.10455035464347441No Hit
CAGTAAAACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10540.10386057850539307No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10540.10386057850539307No Hit
GTCTGGGGGAGGCTTGGTTCAGCCTGGGGGGTCCCTAAGACTCTCATGTCTAGCCTCTGGATTCCCCTTTAGCAA10530.10376203905709573No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10520.10366349960879838No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10520.10366349960879838No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10500.10346642071220372No Hit
GCCGTATATTACTGTGCGAAACCCCTGGTGGGGTTTATTCAACCAACTAG10470.1031708023673117No Hit
CCTCATCTACGGTGCATTTAGAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10440.10287518402241969No Hit
GGACAATGGTCACCGTGTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC10420.10267810512582504No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10380.10228394733263568No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA10300.10149563174625698No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10280.10129855284966231No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10150.10001754002179693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)