FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224714
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43300.3535519313080442No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41070.33534359858709867No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGGTGGCCTTGGGCTGA38840.31713526586615326No Hit
GTGTCCGGGTCTCCTGGACTGTCAGTCACCATCTCCTGCACTGGAACCAG36810.3005599674699563No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33940.2771259249098157No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33570.274104811409031No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGGTGGCCTTGGG33220.27124700134072116No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGG32510.2654497294878641No Hit
GTCCTAGGTCAGCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTC31640.25834603017520824No Hit
GCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31000.2531203203360131No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCCCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG28380.23172757068180816No Hit
GGCCTGGGCTCTGCCCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC27570.22511378166657686No Hit
CTCCTGGACTGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT26660.21768347548897132No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26570.2169486100428345No Hit
GCCCCTATGTGTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC25950.21188620363611424No Hit
GAGTGACAGTGGGGGTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATAGGGGCTGC25700.20984491073017864No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATA25470.2079669212567179No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25330.20682379722939395No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGGC25280.20641553864820683No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG24910.20339442514742218No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24840.2028228631337602No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCAC24800.20249625626881051No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24500.20004670478168782No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACCTTGTCTCCTGGTATCAACAGCACCCAGGCCAAGC24430.19947514276802583No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24220.19776045672703996No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCCGATTATTACTGCTGCTCATATGCAGGCAGCCCCTATGTGTTCGGGA24060.19645402926724118No Hit
GTAGTACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATCTGCAAAAATA23750.19392282606388103No Hit
CATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGGCCTCATCTTCA23640.19302465718526937No Hit
GTTATAACCTTGTCTCCTGGTATCAACAGCACCCAGGCCAAGCCCCCAAG23240.18975858853577243No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCACCATCTCCTG22880.1868191267512252No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22730.18559435100766383No Hit
TCCTAGGTCAGCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21600.17636770707283494No Hit
GGTATCAACAGCACCCAGGCCAAGCCCCCAAGGTCATGATTTATGATGTC21030.1717135592473018No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACCTTGTCTCCTGGTATCAACAGCA19970.1630584773261349No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19940.16281352217742265No Hit
GGCCAAGCCCCCAAGGTCATGATTTATGATGTCACTGAGCGGCCCTCAGG19330.1578327674869398No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19320.1577511157707024No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19100.15595477801347907No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18230.14885107870082323No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18130.148034561538449No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCAC18110.14787125810597412No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGA17530.14313545856420357No Hit
GTAATAATCGGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17420.1422372896855919No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCACCATCT17210.140522603644606No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16980.13864461417114526No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16760.13684827641392194No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16730.13660332126520966No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16180.13211247687215136No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16110.13154091485848943No Hit
CCTGAGGGCCGCTCAGTGACATCATAAATCATGACCTTGGGGGCTTGGCC15780.12884640822265445No Hit
GTGTACTACTGTGCGAGGTATTTTTTGGGTCATTCCTATTTTGACTACTGGGGCCCGGGAACCGTGGTCATCGT15720.12835649792522988No Hit
CCCCTGAGGGCCGCTCAGTGACATCATAAATCATGACCTTGGGGGCTTGGCCTGGGTGCTGTTGATACCAGGAGA15660.12786658762780534No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15380.12558033957315748No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCACCATCTCCTGCA15340.1252537327082078No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15330.12517208099197039No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGGTGGCCTTGGGCTGA15040.12280418122108509No Hit
GTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTC15030.12272252950484767No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14950.12206931577494827No Hit
ACCATGGCCTGGGCTCTGCCCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT14680.11986471943653783No Hit
GTCCCGAACACATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGGC14520.11855829197673906No Hit
GTTGATACCAGGAGACAAGGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACAGTC14420.11774177481436482No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14260.11643534735456605No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG14250.11635369563832863No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT14100.11512891989476726No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14060.11480231302981757No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13990.11423075101615562No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13900.1134958855700188No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13880.11333258213754396No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13840.11300597527259425No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCCCCTATGTGTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAG13600.11104633408289609No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT13570.11080137893418382No Hit
TATGCAGGCAGCCCCTATGTGTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCACCCCCA13440.10973990662309732No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13220.107943568865874No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13210.10786191714963658No Hit
CCCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAAC12740.10402428648647766No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12670.1034527244728157No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12660.10337107275657828No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12600.10288116245915373No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCCGATTATTACTGCTGCTCATATGCAGGCAGCCCCTATGTGTT12480.10190134186430465No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT12440.10157473499935496No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCCCCTCCTCAGCCTCCTC12400.10124812813440526No Hit
GGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACT12400.10124812813440526No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTC12370.10100317298569299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2300.067.362770
ATATCGT200.007362446351.74411837
GTGGTAT2350.051.110681
TCTCGTA1850.046.63546838
TCGTATG2100.046.08368740
ACGAATA306.4868794E-445.7450143
CGTATGC2200.045.6031541
GGCGAAT2300.044.8622932
CTTATAC2550.044.4104541
TAAGGCG2400.044.38071429
ACGAGAC2400.044.31367522
GCGAATC2100.044.2467533
TTATACA2700.043.2036252
ATCTCGT2000.043.120137
GTATGCC2350.042.74334342
GAGACTA2500.042.54807724
CGAGACT2500.042.544623
TATACAC2850.040.9297523
CACGAGA2600.040.90493421
ATGCCGT2400.040.4730544
Produced by FastQC (version 0.11.5)