FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224714
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44870.36637125075731963No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38630.31542057982516736No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGGTGGCCTTGGGCTGA36480.2978654608341213No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35250.2878222997369182No Hit
GTGTCCGGGTCTCCTGGACTGTCAGTCACCATCTCCTGCACTGGAACCAG35210.28749569287196847No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGGTGGCCTTGGG32380.2643882571767776No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31980.26112218852728064No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGG30040.24528175557722046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29800.2433221143875223No Hit
GTCCTAGGTCAGCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTC27940.22813489516736152No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCCCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG27060.22094954413846823No Hit
GCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26890.21956146496243203No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26690.21792843063768358No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25760.2103348210276032No Hit
GGCCTGGGCTCTGCCCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC25700.20984491073017864No Hit
CATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGGCCTCATCTTCA25340.2069054489456314No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCAC25160.20543571805335778No Hit
GAGTGACAGTGGGGGTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATAGGGGCTGC24710.2017613908226737No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23800.19433108464506815No Hit
GCCCCTATGTGTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC23800.19433108464506815No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATA23680.19335126405021907No Hit
CTCCTGGACTGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT23560.19237144345537No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTCCCGAACACATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGGC23520.1920448365904203No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23420.19122831942804605No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23210.18951363338706018No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22870.18673747503498778No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCCGATTATTACTGCTGCTCATATGCAGGCAGCCCCTATGTGTTCGGGA22670.1851044407102393No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCACCATCTCCTG22330.1823282823581669No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACCTTGTCTCCTGGTATCAACAGCACCCAGGCCAAGC22250.1816750686282675No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG21690.17710257251897177No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21580.1762044036403601No Hit
GTAGTACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATCTGCAAAAATA20830.17008052492255335No Hit
GTTATAACCTTGTCTCCTGGTATCAACAGCACCCAGGCCAAGCCCCCAAG20460.1670594114217687No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19940.16281352217742265No Hit
GGTATCAACAGCACCCAGGCCAAGCCCCCAAGGTCATGATTTATGATGTC19050.15554651943229195No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18590.15179054048537047No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACCTTGTCTCCTGGTATCAACAGCA18260.1490960338495355No Hit
TCCTAGGTCAGCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17980.14680978579488763No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17790.1452584031863766No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17430.14231894140182932No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16950.13839965902243298No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16870.1377464452925336No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16870.1377464452925336No Hit
GGCCAAGCCCCCAAGGTCATGATTTATGATGTCACTGAGCGGCCCTCAGG16780.1370115798463968No Hit
GTAATAATCGGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16330.13333725261571275No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15990.13056109426364032No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCAC15870.12958127366879124No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGA15830.12925466680384154No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCACCATCT15780.12884640822265445No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACTGTCAGTCACCATCTCCTGCA15300.1249271258432581No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15090.1232124398022722No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14840.12117114689633661No Hit
CCCCTGAGGGCCGCTCAGTGACATCATAAATCATGACCTTGGGGGCTTGGCCTGGGTGCTGTTGATACCAGGAGA14840.12117114689633661No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14710.12010967458525011No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14440.11790507824683968No Hit
GTGTACTACTGTGCGAGGTATTTTTTGGGTCATTCCTATTTTGACTACTGGGGCCCGGGAACCGTGGTCATCGT14390.11749681966565255No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14370.11733351623317771No Hit
ACCATGGCCTGGGCTCTGCCCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT14280.11659865078704089No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGGTGGCCTTGGGCTGA14270.11651699907080347No Hit
CCTGAGGGCCGCTCAGTGACATCATAAATCATGACCTTGGGGGCTTGGCC14190.11586378534090408No Hit
GTTGATACCAGGAGACAAGGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACAGTC14180.11578213362466666No Hit
GTCCCGAACACATAGGGGCTGCCTGCATATGAGCAGCAGTAATAATCGGC14120.11529222332724212No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13760.11235276154269486No Hit
GTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCACCCCCACTGTCACTCTGTTCCCGCCCTC13680.11169954781279548No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13270.10835182744706111No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG13220.107943568865874No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13150.10737200685221203No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT13150.10737200685221203No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12820.10467750021637705No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12790.10443254506766478No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12680.10353437618905312No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12660.10337107275657828No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12660.10337107275657828No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12580.10271785902667889No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT12480.10190134186430465No Hit
TATGCAGGCAGCCCCTATGTGTTCGGGACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCACCCCCA12390.10116647641816782No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCCGATTATTACTGCTGCTCATATGCAGGCAGCCCCTATGTGTT12330.1006765661207433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG200.007587496751.351567
GTGGTAT2900.048.433941
TCTGGAG8200.038.93618470
GTATAGC451.0439626E-438.0646131
GAACCCG500.002831426233.8949569
ATCAACG35900.031.179023
GTACGAC450.00480379730.4268253
TCAACGC36950.030.2003754
CAACGCA37300.029.9182155
AACGCAG37900.029.6276386
ACGCAGA38700.029.0151797
CGCAGAG39300.028.5721998
AGAGTAC40050.027.8661811
CAGAGTA40650.027.5390910
GCAGAGT40850.027.4880649
TAGTACA7300.026.7287652
AGTACAC7350.026.5458533
GTTGTCT7650.026.4822264
ATGGCGT7500.026.32261359
CGTTGTC7450.026.19680663
Produced by FastQC (version 0.11.5)