FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005893
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62280.619151341146623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61850.614876532593427No Hit
ATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT56020.5569180817442809No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTACAGTTCCGACAATAAGAACTA46210.4593927982399718No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41560.41316521737401496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37390.37170951582325357No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34890.3468559777232767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32050.31862235844170306No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31860.3167334895461048No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31510.31325399421210803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31120.3093768422685117No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31120.3093768422685117No Hit
GTTTTCTACAGTTCCGACAATAAGAACTACCTTGCTTGGTTCCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTC30980.30798504413491296No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29840.2966518307613235No Hit
GTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA28270.28104380883453806No Hit
GTTTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA28130.27965201070093937No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28000.27835962671974057No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26610.2645410595361534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26460.26304984725015484No Hit
CTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT26320.2616580491165561No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC26130.25976918022095785No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25950.25797972547775955No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25200.2505236640477665No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTA24990.24843596684736846No Hit
ATTTAGGAGGCTGCCCTGGCTTCTGCTGGAACCAAGCAAGGTAGTTCTTA24620.24475764320857188No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA23890.23750041008337866No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC23390.23252970246338328No Hit
CTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22450.223184772137792No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22400.22268770137579244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22270.22139531739459364No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21720.21592753901259876No Hit
ACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT21570.21443632672660012No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGT21050.20926679080180496No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20330.20210897182901164No Hit
GAACTACCTTGCTTGGTTCCAGCAGAAGCCAGGGCAGCCTCCTAAATTGC20300.2018107293718119No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20100.19982244632381377No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18910.1879921621882248No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18790.1867991923594259No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18320.18212672719663026No Hit
AGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAA18090.1798402016914324No Hit
ACCTTGCTTGGTTCCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTT18060.17954195923423266No Hit
GTAATACACAGCCGTGTCCTCGGCCCCCAGACTGTTCATCTGCAGATACA17880.17775250449103433No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC17620.17516773652863674No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17260.1715888270422401No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG17130.17029644306104127No Hit
GTTCCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA17070.16969995814664185No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16900.1680099175558434No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16560.16462983637424655No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16480.16383452315504732No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAAGGAGCAATTTAGGAGGCTGCCCTGGCTTCTGCTGGAA16470.1637351090026474No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16380.16284038163104825No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16230.16134916934504961No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15710.15617963342025443No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15680.1558813909630547No Hit
GTAGAAAACACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGAGTCTGGAGA15480.1538931079150566No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15470.15379369376265667No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15450.15359486545785686No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15450.15359486545785686No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15320.15230248147665806No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14890.14802767292346203No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14870.14782884461866222No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14690.1460393898754639No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14490.14405110682746575No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14480.14395169267506586No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTT14420.1433552077606664No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGG14370.14285813699866687No Hit
GACTACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14290.1420628237794676No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14040.1395774699694699No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13920.13838450014067102No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13820.13739035861667195No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13810.13729094446427204No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13730.1364956312450728No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT13720.13639621709267288No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG13680.13599856048307327No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTG13670.13589914633067335No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13560.13480559065427436No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13420.13341379252067567No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGTCCGC13370.1329167217586761No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13240.1316243377774773No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13140.13063019625347824No Hit
GTGTATTACTGTTCAAGAGGGGATGGGCCGCTCTGGCACCTCAATCTCTGGGGCCGTGGCACCCTAGTCA13100.13023253964387863No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13060.12983488303427898No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13040.12963605472947917No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12890.12814484244348057No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT12870.12794601413868076No Hit
CTACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12870.12794601413868076No Hit
AGGTAGTTCTTATTGTCGGAACTGTAGAAAACACTCCGGCTGGACTTGCA12770.1269518726146817No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12680.1260571452430825No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12620.12546066032868308No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12330.12257764990908576No Hit
TTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGA12020.11949581118468862No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11980.119098154575089No Hit
CCCAGAGACAGAGCCAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCC11950.11879991211788928No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11810.11740811398429057No Hit
CTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTACAGTTCCGA11790.11720928567949077No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC11550.11482334602189298No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11300.1123379922118953No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG11290.1122385780594954No Hit
GCCCTGGCTTCTGCTGGAACCAAGCAAGGTAGTTCTTATTGTCGGAACTG11260.11194033560229567No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11030.1096538100970978No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG10960.10895791103029845No Hit
GCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT10930.10865966857309872No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10930.10865966857309872No Hit
CTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCA10880.10816259781109919No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA10790.10726787043950003No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10710.10647255722030077No Hit
GGCTTCTGCTGGAACCAAGCAAGGTAGTTCTTATTGTCGGAACTGTAGAA10590.10527958739150187No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10500.10438486001990271No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10280.10219774866710475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT252.5946836E-455.0803439
CTGACGA200.00744130951.60417638
CTACGCT3100.050.72131728
GTGGTAT2250.050.0534741
TCGTATG2100.049.21096840
TACGCTG3200.049.1607629
ACTACGC3250.048.3610627
CACGAGA3400.048.1822121
TTATACA3150.047.6557542
GAGACTA3400.047.1854524
TGCGTGA604.6875357E-946.94336755
TGGTATC2350.046.457452
CGTATGC2600.046.427841
CTTATAC3250.046.2032051
GTATGCC2650.045.609142
TATACAC3350.044.810643
GGTATCA7700.044.3212551
ACGCTGC3650.044.0587530
ACGAGAC3650.043.94482822
ATGCCGT2650.043.1113244
Produced by FastQC (version 0.11.5)