FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860112
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29060.337862975984523No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27190.31612162137024014No Hit
GTTTATTACTGCATGGCAGCTACACAATTAGGCATTTTTGGCCAGGGGAC26210.30472775638521493No Hit
CTCCTACACAGTGATGGAAACACCTACTTGAGTTGGTTCCAGCAGAAGCC24820.2885670703350261No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT21510.2500837100284614No Hit
GTAATAAACCGCGACATCGTCTGTTTGCACCCTGCTGATCTGCAGTGTGA18690.21729728221440928No Hit
CCCCACACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA18610.2163671707870603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18300.2127629890060829No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16500.19183548189073052No Hit
CAGTAATATAGAGCCGTGTCGTCGACTCTCAGACTGTCCATTTGGAGAAA16220.18858009189500902No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16030.18637107725505514No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15910.18497591011403167No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCACAGCCTCCTA15540.18067414476254257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15520.1804416169057053No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15510.18032535297728666No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14090.16381587514184198No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13680.1590490540766784No Hit
GTGATGGAAACACCTACTTGAGTTGGTTCCAGCAGAAGCCAGGCCAGCCTCCAAGACTCCTAATTTATCAGGTCT13350.15521234443886378No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12770.14846903659058355No Hit
GTGTAGGAGGCTGTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACAGGTGTGGGGAGTGCAGG12580.1462600219506297No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT12150.14126067302862882No Hit
ATATTACTGTGTGCGAGACCGGAGTGGCTGGCCCTTTGACTTCTGGGGCC12100.1406793533865357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12020.1397492419591867No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11840.13765649124765147No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11710.13614506017820935No Hit
GACTTCACACTGCAGATCAGCAGGGTGCAAACAGACGATGTCGCGGTTTA11030.1282391130457429No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10960.12742526554681252No Hit
GTCCCAGACAGATTCACTGGCAGTGGGGCAGGGACAGACTTCACACTGCA10900.12672768197630077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10850.12614636233420762No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10740.12486745912160277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10730.12475119519318414No Hit
GTCTGGGACCCCAGATAATCGCTGAGAGACCTGATAAATTAGGAGTCTTGGAGGCTGGCCTGGCTTCTGCTGGAA10530.12242591662481166No Hit
GTAATATAGAGCCGTGTCGTCGACTCTCAGACTGTCCATTTGGAGAAATA10520.12230965269639303No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10250.11917052662909015No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA10190.1184729430585784No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10110.11754283163122942No Hit
CTGCAGATCAGCAGGGTGCAAACAGACGATGTCGCGGTTTATTACTGCAT10100.11742656770281079No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG10060.1169615119891363No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9690.11265974663764719No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9280.10789292557248358No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTG9140.10626523057462284No Hit
CCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCT9130.10614896664620421No Hit
AGCCTGCACTCCCCACACCTGTCACCCTTGGACAGCCGGCCTCCATCTCC9130.10614896664620421No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9000.10463753557676209No Hit
GTGTGAAGTCTGTCCCTGCCCCACTGCCAGTGAATCTGTCTGGGACCCCA8820.10254478486522685No Hit
AATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTC8750.10173093736629649No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8750.10173093736629649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.048.4880561
CGTATGC5350.040.09917441
ATCTCGT4800.040.07109537
TCGTATG5350.040.0325840
ATATCGT350.001377453339.25331537
ATGCCGT5450.038.897244
TATACAC5600.038.0918963
CTTATAC5700.037.441031
AATCTCG5050.037.3521836
AAAGGGG5200.036.74416468
GTATCAA26200.036.7197041
CCCACGA5550.036.61546719
GGCAATC5150.036.45516633
ATCTCCG5800.036.19880710
GTATGCC6050.036.0915642
CACGAGA5650.035.97368221
TCTCCGA5950.035.28623211
TCCGAGC5950.035.2821313
CGTCTTC6100.035.0325148
CGAGCCC5900.035.0133515
Produced by FastQC (version 0.11.5)