FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860112
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41420.4815651915099429No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26870.31240117566084413No Hit
GTTTATTACTGCATGGCAGCTACACAATTAGGCATTTTTGGCCAGGGGAC25100.2918224603307476No Hit
CTCCTACACAGTGATGGAAACACCTACTTGAGTTGGTTCCAGCAGAAGCC25040.2911248767602359No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24590.2858929999813978No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19260.22392432613427088No Hit
GTAATAAACCGCGACATCGTCTGTTTGCACCCTGCTGATCTGCAGTGTGA17960.2088100154398497No Hit
CCCCACACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA17310.2012528600926391No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15130.17590732369737894No Hit
CAGTAATATAGAGCCGTGTCGTCGACTCTCAGACTGTCCATTTGGAGAAA14670.1705591829901222No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14550.1691640158490987No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14410.16753632085123796No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13710.15939784586193426No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCACAGCCTCCTA13590.15800267872091076No Hit
ATATTACTGTGTGCGAGACCGGAGTGGCTGGCCCTTTGACTTCTGGGGCC12690.14753892516323455No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12540.14579496623695518No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12390.14405100731067583No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12330.14335342374016408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11950.13893539446025632No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11740.13649385196346522No Hit
GTGTAGGAGGCTGTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACAGGTGTGGGGAGTGCAGG11680.13579626839295347No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT11650.1354474766076976No Hit
GTGATGGAAACACCTACTTGAGTTGGTTCCAGCAGAAGCCAGGCCAGCCTCCAAGACTCCTAATTTATCAGGTCT11530.13405230946667412No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.13265714232565062No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11370.1321920866119761No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA11260.13091318339937125No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11230.13056439161411537No Hit
GTGTGAAGTCTGTCCCTGCCCCACTGCCAGTGAATCTGTCTGGGACCCCA11230.13056439161411537No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11200.1302155998288595No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11100.12905296054467325No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10920.126960209833138No Hit
CTGCAGATCAGCAGGGTGCAAACAGACGATGTCGCGGTTTATTACTGCAT10790.1254487787636959No Hit
GTCTGGGACCCCAGATAATCGCTGAGAGACCTGATAAATTAGGAGTCTTGGAGGCTGGCCTGGCTTCTGCTGGAA10450.12149580519746266No Hit
GACTTCACACTGCAGATCAGCAGGGTGCAAACAGACGATGTCGCGGTTTA10340.12021690198485778No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10090.11731030377439217No Hit
GTCCCAGACAGATTCACTGGCAGTGGGGCAGGGACAGACTTCACACTGCA9840.11440370556392657No Hit
GTAATATAGAGCCGTGTCGTCGACTCTCAGACTGTCCATTTGGAGAAATA9520.11068325985453056No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9440.10975314842718156No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9430.10963688449876295No Hit
AATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTC9010.10475379950518073No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG8890.10335863236415722No Hit
CTATATTACTGTGTGCGAGACCGGAGTGGCTGGCCCTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTC8720.10138214558104061No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8710.101265881652622No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTG8660.10068456201052886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.043.4628331
GTATCAA27250.037.9184881
TGGTATC3050.036.5095982
GTGGTAT3300.034.790531
TATCAAC30650.033.3583952
ATCAACG31500.032.5615843
TCAACGC31600.032.353654
CAACGCA32100.031.8552535
AACGCAG32000.031.8530436
CGTCTCC4200.031.323167
ACGCAGA33850.030.1121837
CGCAGAG34450.029.7836788
AGAGTAC34600.029.65455811
GTTAGGT803.2469961E-629.5652920
GCAGAGT35300.029.2577349
CAGAGTA35150.029.09452410
GGTAACG659.837489E-425.9627466
CGACCGG2500.025.65119411
GTGCTAG1751.0913936E-1125.0842631
CTAGAGA1501.3478711E-924.7439584
Produced by FastQC (version 0.11.5)