FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346690
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCCATAGGCGGATGTTGTAAAGGTGGCAGGTGCTACTC8560.2469064582191583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8380.2417144999855779No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGAAGATACA7960.2295999307738902No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6620.19094868614612479No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5880.16960396896362745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5730.1652773371023104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5670.1635466843577836No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5590.16123914736508121No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5500.15864316824829097No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5470.15777784187602759No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5420.15633563125558855No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5360.15460497851106175No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5220.15056678877383253No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5210.15027834664974474No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4990.1439326199198131No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4770.13758689318988146No Hit
GTGTGAGGTGCGCCTGGTGGAGTCTGGGGGAGGCTTCGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC4750.13701000894170587No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4730.13643312469353025No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4660.13441402982491563No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4560.13152960858403762No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC4420.1274914188468084No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4360.12576076610228157No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4120.11883815512417432No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4110.11854971300008653No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG4080.11768438662782313No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC3960.1142230811387695No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG3860.11133865989789149No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC3790.10931956502927688No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3750.10816579653292566No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3730.10758891228475007No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG3660.10556981741613544No Hit
CTCCTGTGCAGCCTCTGGATTCTCCGTCAGAGACACCGACGTGGCCTGGGTCCGCCGGGCTCCAGGGAAGGGG3620.10441604891978426No Hit
CAGTAATACTTGGAGTAGCACCTGCCACCTTTACAACATCCGCCTATGGC3520.10153162767890622No Hit
GTATAACGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCAT3490.10066630130664282No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3480.10037785918255503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCG200.006605561453.17468653
CGTATTT304.8817683E-448.44987563
ATGCCGT1000.045.21880344
CGTATGC1000.044.99247441
TCGTATG1000.044.91313640
ATCTCGT950.043.39795737
GTATCAA10600.042.252821
AGCCCAC1050.041.984717
CCCACGA1050.041.984719
CTTATAC1150.041.3064461
TACGGAG2800.040.19618270
CTGATCT1050.039.11619634
CACGAGA1150.038.35598421
ACGAGAC1150.038.35598422
TCTCCGA1150.038.3338511
GCCGTCT1200.037.8334746
GTATGCC1200.037.5600842
CGAGGCT1100.037.08444629
GCCCACG1200.036.7366118
TATCAAC12000.036.454022
Produced by FastQC (version 0.11.5)