FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346690
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13200.38074360379589833No Hit
GTGTATTACTGTGCCATAGGCGGATGTTGTAAAGGTGGCAGGTGCTACTC8350.24084917361331448No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGAAGATACA7370.2125818454527099No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7190.20738988721912946No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6260.1805647696789639No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6010.17335371657676887No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5330.15373965213879834No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4970.14335573567163748No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4800.13845221956214485No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4740.13672156681761805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4690.13527935619717904No Hit
GTGTGAGGTGCGCCTGGTGGAGTCTGGGGGAGGCTTCGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC4680.13499091407309124No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4490.129510513715423No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4390.12662609247454498No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4170.12028036574461334No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4110.11854971300008653No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC3990.11508840751103293No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3910.11278087051833048No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3880.1119155441460671No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3840.1107617756497159No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA3830.11047333352562808No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC3820.11018489140154028No Hit
CTCCTGTGCAGCCTCTGGATTCTCCGTCAGAGACACCGACGTGGCCTGGGTCCGCCGGGCTCCAGGGAAGGGG3710.10701202803657446No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3660.10556981741613544No Hit
GTATAACGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCAT3510.10124318555481843No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT3500.10095474343073062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC150.002405118468.6280936
TTAACGG150.00242192868.50767535
TCATCGT350.00508940856.78619870
TGCGAAC200.005701569455.18972866
TGATAGT200.00681648352.7537949
TCGCAGT305.842315E-446.7169247
TAATATC306.848245E-445.2352427
GTGGTAT851.8189894E-1243.7843131
GTATCAA10350.038.2463761
GCTTGTA502.0845154E-433.8333321
ACAGGTA701.1310512E-633.8333321
AATGCGG553.10286E-431.61601343
AAATGCG553.1381584E-431.55511342
TTAGGGA450.004398750630.9732144
TACGGAG3000.030.91693170
TATCAAC12800.030.890092
GGACTGA553.6682E-430.7265156
GGTATCA6450.030.4237731
CCCGTGA604.1245876E-430.11699161
GTGTGTA450.00508789730.0653923
Produced by FastQC (version 0.11.5)