FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988039
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54700.5536218712014404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52100.5273071204679167No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38020.3848026241879116No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36900.37346703925654756No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34950.3537309762064048No Hit
ATATTATCTTACTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT34500.34917650011791035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32640.3303513322854665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32350.32741622547288113No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31360.31739637807819326No Hit
GTTTGGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGGGAATAAAAGACT30580.30950195285813614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30240.3060607931468292No Hit
CTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT29940.30302447575449953No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29370.2972554727090732No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATT29210.29563610343316404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29190.29543368227367545No Hit
CTTTTATTCCCCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA28460.28804530995233996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27920.28257993864614656No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27320.27650730386148725No Hit
AGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAA27220.27549519806404404No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26920.27245888067171437No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGGG25740.2605160322618844No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24570.24867439443179876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23740.24027391631302003No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22920.23197464877398566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22440.2271165409462582No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC22230.22499111877162742No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21630.21891848398696814No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21580.2184124310882465No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21150.21406037615924067No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATCTTACT20420.20667200383790518No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19810.20049815847350155No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19680.19918242093682537No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCCCCTCCAA18770.18997225818009209No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTGGGAGGCTGTCCTGGCTTCTGCTGGTA18310.18531657151185327No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18280.18501293977262032No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18000.1821790435397793No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17380.17590398759563136No Hit
ATCTTACTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT16980.17185556440585847No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16810.170134984550205No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16620.1682119835350629No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16590.1679083517958299No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16460.16659261425915373No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16340.1653780873022219No Hit
AGATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16170.1636575074465684No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15960.16153208527193763No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15960.16153208527193763No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15800.1599127159960285No Hit
GTTTATTTCTGTCACCAATATTATCTTACTCCGACATTCGGCCAAGGGAC15570.1575848726619091No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT15490.15677518802395451No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15420.15606671396574426No Hit
GTAAATGAGCAGTTTGGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTA15420.15606671396574426No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15300.15485218700881243No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15210.15394129179111352No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15190.15373887063162486No Hit
GAATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA15000.15181586961648275No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCCCCTCCAACAATAAGAACTA14940.15120860613801682No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14940.15120860613801682No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14880.15060134265955089No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14630.14807107816594284No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATCTTACTCCGACATTCGG14600.14776744642670986No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14550.14726139352798825No Hit
CTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATC14410.14584444541156777No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14310.14483233961412456No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14310.14483233961412456No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14230.14402265497616998No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14100.1427069174394938No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14080.14250449628000514No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13640.13805123077125497No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13500.13663428265483446No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13370.1353185451181583No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13350.13511612395866965No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13280.1344076499004594No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATCTTACTCCGA13250.1341040181612264No Hit
CTACGGGGACATCGTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13220.13380038642199346No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13070.13228222772582865No Hit
GTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA13010.13167496424736272No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12970.1312701219283854No Hit
GAGTAAGATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12850.13005559497145355No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA12730.1288410680145217No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12730.1288410680145217No Hit
GAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGC12310.12459022366526017No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12270.12418538134628287No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC12120.12266722265011808No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12050.1219587485919078No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11920.12064301105523163No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11860.1200357475767657No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11770.11912485235906681No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11670.11811274656162359No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11540.11679700902494738No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11530.11669579844520307No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG11520.11659458786545875No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11400.1153800609085269No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGG11380.11517763974903823No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11200.11335584931364046No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11200.11335584931364046No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11100.11234374351619723No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC10960.11092679539977672No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10950.11082558482003241No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT10900.11031953192131079No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10870.11001590018207784No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTAC10830.10961105786310055No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10820.10950984728335623No Hit
ACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGCTCATT10790.10920621554412326No Hit
CCTTGGTCCCTTGGCCGAATGTCGGAGTAAGATAATATTGGTGACAGAAA10740.10870016264540165No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG10420.10546142409358335No Hit
GCTAAGTAGTTCTTATTGTTGGAGGGGAATAAAAGACTCTGGCTGGACTT10410.10536021351383903No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC10350.1047529500353731No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10320.10444931829614013No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10280.10404447597716283No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT10270.10394326539741852No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCGGAGT10020.10141300090381047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA200.00776602451.049612
CGCTAAT3150.048.90081432
TCGTATG3200.047.49007840
GTGGTAT2550.046.7380831
TCGCTAA3300.046.6353231
GCTAATC2800.046.4912733
CGTATGC3350.045.43366641
TTATACA3450.045.377432
TATACAC3450.045.377433
ACACTCG3400.045.14430227
CTTATAC3550.045.0829731
ATCTCGT2900.043.9036737
ACTCGCT3500.043.89929
ACGAGAC3650.042.9170622
ATGCCGT3600.042.4138244
CTAATCT3000.042.2822734
TCTCCGA3650.041.95858411
AATCTCG3050.041.6932736
TACACAT4000.041.6905145
CTCGCTA3700.041.55562230
Produced by FastQC (version 0.11.5)