FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985484
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41790.42405559095835144No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34960.35474954438631173No Hit
GTATCATTGTCACCAGTATGGTAGCTCACCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG27420.27823891610619755No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27100.2749917806884739No Hit
GTATGGTAGCTCACCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT25220.2559148601093473No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23600.23947623705712118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23070.23409816902151634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21930.22253024909587574No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19750.2004091390626332No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18950.192291300518324No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18510.18782648931895393No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18020.18285431321056456No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17320.17575120448429402No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17250.17504089361166697No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC17170.17422910975723604No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15760.15992141932289108No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15690.15921110845026404No Hit
CAATGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15680.1591096354684602No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15560.15789195968681377No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15080.15302125656022827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14870.1508903239423471No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGA14820.1503829590333278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14750.14967264816070075No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14610.14825202641544663No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAGCAG14590.1480490804518389No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14260.14470047205231137No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCAGTGGCCCTGGT13960.1416562825981954No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13910.14114891768917606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13740.1394238769985104No Hit
CTACCATACTGGTGACAATGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC13520.13719147139882534No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGGACAT13460.13658263350800215No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13450.1364811605261983No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13190.13384286299929782No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13140.1333354980902785No Hit
GTGTATCATTGTCACCAGTATGGTAGCTCACCGACGTTCGGCCAAGGGAC13130.1332340251084746No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13050.1324222412540437No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12490.12673975427302725No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12040.12217347009185334No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12020.12197052412824562No Hit
GTAATAAATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG11910.1208543213284031No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATCATTGTCACCAGTATGGTAGCTCA11690.11862191572871808No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11460.11628803714722917No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11230.11395415856574027No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10960.11121438805703594No Hit
TCTATGGGACATCCACCAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT10900.11060555016621275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10500.10654663089405814No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCA10450.10603926598503882No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10200.1035024414399422No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10170.10319802249453061No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10130.10279213056731515No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9970.10116856285845331No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9960.10106708987664943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29750.037.9767341
CCGTTAA656.387818E-736.4082816
GGTATCA15850.033.9332921
TTGCGCC400.002879580333.7785264
TATCAAC34500.032.9056972
ATCAACG36100.031.2521573
TCAACGC36400.030.9017854
ATAACTC553.6862394E-430.7077523
CAACGCA37000.030.4022165
GTCGCTT450.005068714730.09406924
AACGCAG37400.029.9897846
TCAGGTC4750.029.87339212
TAGGTGG4600.029.374125
CGCAGAG39450.028.6026528
ACGCAGA39500.028.5664447
GCCGTTA855.187636E-627.838815
AGAGTAC40200.027.73286411
GCAGAGT40950.027.3074369
GTCAGGT5200.027.2868111
ACAGGTA1005.959355E-727.0612681
Produced by FastQC (version 0.11.5)