FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077510
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102560.9518241130012715No Hit
GTAATATACAGCGGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA61780.5733589479447987No Hit
GTATATTACTGTGCGAGAGATCGGGAGGTGGTTACTAAAAACTTCTTTGA49770.4618982654453323No Hit
GGCTAACAGTGTCCCTCAGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT37050.3438483169529749No Hit
CTGTTAGCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC36300.33688782470696327No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32490.3015285240972242No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29370.2725728763538157No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24740.22960343755510387No Hit
GTGTTAGCACCTGGCTAGCCTGGTATCAGCAGAAATTAGGGAAAGCCCCT21840.20268953420385888No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18110.16807268610036102No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18050.1675158467206801No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17060.1583279969559447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16880.15665747881690192No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16580.15387328191849728No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATAGCATAAATTGGGTCCGCCAGGCTCCAGGGAAGGGG16100.14941856688104982No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15970.14821208155840782No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15960.14811927499512764No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15810.14672717654592532No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15800.14663436998264517No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15750.14617033716624442No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTGTTAGCAC15020.13939545804679307No Hit
GTTTAGGGGCTTTCCCTAATTTCTGCTGATACCAGGCTAGCCAGGTGCTA14820.13753932678118996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14690.13633284145854796No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14600.13549758238902654No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14420.13382706424998375No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14390.13354864456014331No Hit
CCCCTAAACTCCTCATCTCTTCTGCATCCATTTTGCAAAGTGGGGTCCCA14380.13345583799686314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14350.13317741830702268No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13840.12844428357973475No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13700.1271449916938126No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13570.12593850637117057No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13210.122597470093085No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13180.12231905040324452No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13140.1219478241501239No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12890.11962766006812No Hit
GATCAGCACTGAGCACCGAGCGATTCACCATGGAACTGGGGCTCCGCTGG12780.11860678787203832No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12740.11823556161891768No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12710.11795714192907722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12470.11572978441035349No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12330.11443049252443133No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12310.11424487939787102No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12260.11378084658147025No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC12190.11313120063850916No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11990.11127506937290606No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11880.11025419717682435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCTGAGGGACA11760.10914051841746249No Hit
GCACTGAGCACCGAGCGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTC11690.10849087247450138No Hit
GTATATTACTGTGCGAGAGATCGGGAGGTGGTTACTAAAAACTTCTTTGACTCCTGGGGCCAGGGAACCCTGGT11670.1083052593479411No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11640.10802683965810062No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11330.10514983619641582No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11280.10468580338001503No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11180.10375773774721347No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGGCTAACAGTGTC11000.1020872196081707No Hit
ACTTACTACTGTCAACAGGCTAACAGTGTCCCTCAGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA10880.10097354084880883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG15000.069.9155870
GTTTTCG200.006528853453.33993551
AGCGGTG13500.043.9572710
CGGCTCT13400.043.56908821
GTGTCGT13600.043.38753514
TCGTCGG13750.042.9361417
GTCGGCT13800.042.78255519
TGTCGTC13750.042.68227415
CGGTGTC14000.042.3873712
TATACAG14250.042.3436625
GGTGTCG13950.042.29699313
CAGCGGT14200.042.262579
GCGGTGT14050.042.23652311
TCGGCTC14100.042.11411320
ACAGCGG14350.042.0544368
GCTCTCA13900.042.03113623
ATACAGC14400.041.9084136
GTTGTCT15050.041.62137264
ATATACA14500.041.6135984
CGTCGGC14200.041.57741518
Produced by FastQC (version 0.11.5)