FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714879
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATGCTGAGAGTGAAATCTGTC36510.5107157994569711No Hit
GTATTACTGTGTGAGAGGTGGGGACTGGAAGTCTCCCCCGGGGCGTGGGGACTACTGGGGCCAGGGAACCCTGGT24570.34369452732560335No Hit
CAGTAATACAGAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGGTA22460.31417904288697807No Hit
ATATAGGACTTACCCGTACAGTTTTGGCCAGGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT21650.3028484540740461No Hit
AATATATACTATGCAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAGCACGCTGTACCTG20570.28774100232347016No Hit
GTAATACAGAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGGTACA19650.27487169157297947No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16760.23444526975893826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15930.2228349133209956No Hit
GTATATATTAGTTCTGTCATATGGAATGAGTGCCACCCACTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG15910.2225551456959849No Hit
CTGTATTACTGTGTGAGAGGTGGGGACTGGAAGTCTCCCCCGGGGCGTGG13510.18898303069470496No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGCCAGTGGATCTGGGACAGATTTCA13050.18254837531945967No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC12540.1754143008816877No Hit
GTAGGAGACAGGGTCACCATCACTTGTCGGGCGAGTCATGACATTAGCAACTATTTAGCCTGGTTTCAGCAGAAA12450.17415534656913967No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11920.16674150450635702No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11830.16548255019380903No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11760.1645033635062717No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCGTAGTTAC11270.15764905669351037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10930.15289300706832906No Hit
GGGCTAGAGTGGGTGGCACTCATTCCATATGACAGAACTAATATATACTA10850.1517739365682864No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10700.1496756793807064No Hit
CTGTAGGAGACAGGGTCACCATCACTTGTCGGGCGAGTCATGACATTAGC10680.14939591175569572No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10490.14673811931809438No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10160.1421219535054184No Hit
GTTTTGGCCAGGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCA9830.1375057876927424No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGGGTCACCATCACTTGTCGGGCGAGTCATGACATTAGCAA9240.12925264275492776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9190.12855322369240108No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC9180.12841333987989575No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC9180.12841333987989575No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9050.12659485031732642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8980.1256156636297891No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8960.12533589600477843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8820.12337752262970376No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8740.1222584521296611No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT8720.12197868450465044No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8690.12155903306713443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8530.11932089206704911No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8500.11890124062953311No Hit
CTCCTGTGCAGCCTCCGGATTCATCTTCAGTAACTACGCTATGCACTGGG8380.11722263487946913No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8100.11330588812931978No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT8080.11302612050430913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8070.11288623669180378No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8020.11218681762927712No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8020.11218681762927712No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7920.1107879795042238No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7910.11064809569171846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7790.10896948994165447No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7780.10882960612914913No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA7760.10854983850413846No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT7720.10799030325411713No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7700.10771053562910647No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT7520.10519262700401047No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7500.1049128593789998No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7340.10267471837891447No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC7300.10211518312889314No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG7270.10169553169137714No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7220.10099611262885048No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7190.10057646119133448No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT7190.10057646119133448No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7150.10001692594131313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)