FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714879
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATGCTGAGAGTGAAATCTGTC43640.6104529577732735No Hit
GTATTACTGTGTGAGAGGTGGGGACTGGAAGTCTCCCCCGGGGCGTGGGGACTACTGGGGCCAGGGAACCCTGGT26190.3663557049514673No Hit
ATATAGGACTTACCCGTACAGTTTTGGCCAGGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT23100.3231316068873194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21850.3056461303241528No Hit
AATATATACTATGCAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAGCACGCTGTACCTG21640.3027085702615408No Hit
CAGTAATACAGAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGGTA21430.29977101019892877No Hit
GTAATACAGAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGGTACA17540.24535620713435422No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16620.23248689638386355No Hit
GTATATATTAGTTCTGTCATATGGAATGAGTGCCACCCACTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG16470.23038863919628358No Hit
CTGTATTACTGTGTGAGAGGTGGGGACTGGAAGTCTCCCCCGGGGCGTGG13760.1924801260073383No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13420.187724076382157No Hit
GGGCTAGAGTGGGTGGCACTCATTCCATATGACAGAACTAATATATACTA11950.16716115594387304No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC11810.16520278256879836No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGCCAGTGGATCTGGGACAGATTTCA11440.16002708150610104No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10970.15345254231835037No Hit
GTAGGAGACAGGGTCACCATCACTTGTCGGGCGAGTCATGACATTAGCAACTATTTAGCCTGGTTTCAGCAGAAA10930.15289300706832906No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCGTAGTTAC10650.14897626031817973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10630.14869649269316906No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9840.13764567150524773No Hit
CTGTAGGAGACAGGGTCACCATCACTTGTCGGGCGAGTCATGACATTAGC9480.13260985425505575No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9430.13191043519252907No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9300.13009194562995974No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9170.12827345606739043No Hit
GTTTTGGCCAGGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCA8960.12533589600477843No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8940.12505612837976776No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGGGTCACCATCACTTGTCGGGCGAGTCATGACATTAGCAA8770.12267810356717711No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC8710.1218388006921451No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8690.12155903306713443No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8650.12099949781711311No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8620.12057984637959711No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC8560.11974054350456512No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7900.11050821187921311No Hit
CTCCTGTGCAGCCTCCGGATTCATCTTCAGTAACTACGCTATGCACTGGG7870.11008856044169714No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7840.10966890900418114No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7650.1070111165665798No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA7610.10645158131655846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7470.10449320794148381No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT7300.10211518312889314No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7200.10071634500383982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTAT400.0087855749.47475470
GGTATCA7950.043.1411171
GTATCAA21050.037.4259641
CGAGCTT500.00275686934.0774969
TATCAAC23200.033.9267352
GTGGTAT2550.033.2917751
TCTGGAG8700.032.60405370
CAACGCA24300.032.530575
TCAACGC24700.032.0037574
ATCAACG24600.031.9959433
AATCTGT12000.031.92394868
AACGCAG25050.031.6942566
ACGCAGA25700.030.8926477
CGCAGAG26200.030.5620968
AGAGTAC26350.030.51688411
CAGAGTA27150.029.49270410
AGAGTGA12450.029.4472661
TGGTATC2800.029.0800552
GGGGCTA6150.028.96083370
CCGATGC12350.028.8600252
Produced by FastQC (version 0.11.5)