FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662592
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26660.4023592195498889No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24900.37579687047232685No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22130.33399135516275474No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTAGCGG17430.26305780933062883No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15110.22804380372838792No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14840.2239688979039892No Hit
GCCTAGAGCCTGACGATTCTGGATTTTATTACTGTCAGCAGCGTGGTTAC14820.22366705302810777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14460.21823384526224282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14230.21476262918960687No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGCCTAGAGCCTGACGATT14070.2123478701825558No Hit
CTCCTGCAGGGCCAGTCAGACTTTTAGCGGCGGCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCT13920.21008403361344538No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13640.20585820535110594No Hit
GTCCCAGACCCACTGCCAGCGAACCTGGCTGGGATGCCAGTGGCCCTAGT13440.2028397565922921No Hit
CTTTTGGCCAGGGGGCCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA13210.19936854051965613No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCAGC13180.19891577320583406No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13120.19801023857818992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12960.1955954795711388No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12940.1952936346952574No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12500.1886530474258669No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12150.18337076209794262No Hit
TGTATGATGTATCCACTAGGGCCACTGGCATCCCAGCCAGGTTCGCTGGCAGTGGGTCTGGGACAGACTTCAGT11740.1771829421423742No Hit
GAAATACACAGCCGTGTCCTCGACTCTCAGGTTGCTCATGTGAAGATACA11640.17567371776296725No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11590.1749191055732638No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11060.16692021636240703No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11040.16661837148652564No Hit
CTGTTGGTACCAGGCTAAGCCGCCGCTAAAAGTCTGACTGGCCCTGCAGGAGAGGGTGGCACTTTCCCCTGGAGA10980.16571283685888147No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10850.16375084516565247No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTAGCGGCGGCTTAGCCTGGTA10760.16239254322418623No Hit
CCCATGTACACTTTTGGCCAGGGGGCCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTC10500.1584685598377282No Hit
GCTCTAGGCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCAGCGAACCTGGCTGGGATGCCAGTGG10320.1557519559547957No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10300.15545011107891432No Hit
CTCCTGTGCAGCCTCTGGATTCAGCGTCAGTGACATCTACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG10290.15529918864097364No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10240.15454457645127018No Hit
GTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGTGC9780.14760214430599827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9770.14745122186805756No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9750.1471493769921762No Hit
GGCCAAAAGTGTACATGGGAGGCCAGGCGTAACCACGCTGCTGACAGTAA9670.14594199748865064No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGCCCCCTGGCCAAAAGTGTACATGGGAGG9530.14382908335748093No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9530.14382908335748093No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9510.14352723848159954No Hit
GTGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTAGCGGCGGCTTAGCCTGGTACCAACAGAGACCTGGCCAGG9260.1397541775330822No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGCCCCCTGGCCAAAAGTG9230.1393014102192601No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8970.1353774268328021No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8870.13386820245339515No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8870.13386820245339515No Hit
CCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCAGCCAGGTTCGCTGGCAGTGGGTCTGGGACAGACTT8860.13371728001545446No Hit
ACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCAGCCAGGTT8640.1303969863807592No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8600.1297932966289964No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8590.12964237419105573No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8570.12934052931517434No Hit
GTACATGGGAGGCCAGGCGTAACCACGCTGCTGACAGTAATAAAATCCAG8560.12918960687723366No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8540.12888776200135227No Hit
GTGTATTTCTGTGCGAGAGCGGCAGCGGGAACTGAAGGCGTCTACTACTA8430.12722761518400463No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT8230.12420916642519077No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8080.12194532985608036No Hit
CATCATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGCCGCCGCTAAAAGTCTGA8070.12179440741813968No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7940.11983241572491064No Hit
CTGCTACTCTGGCTCCCAGATACCACCGAAGAAGTTGTGTTGACACAGTC7910.11937964841108857No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC7880.1189268810972665No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7710.1163611996522747No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7680.11590843233845263No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGC7660.11560658746257123No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA7600.11470105283492707No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7600.11470105283492707No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7590.11455013039698637No Hit
GGTTACGCCTGGCCTCCCATGTACACTTTTGGCCAGGGGGCCAAGCTGGA7580.11439920795904569No Hit
GACTTCAGTCTCACCATCAGCAGCCTAGAGCCTGACGATTCTGGATTTTATTACTGTCAGCAGCGTGGTTACGC7560.1140973630831643No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7530.11364459576934222No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAAT7270.10972061238288418No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT7140.10775862068965517No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT7080.10685308606201102No Hit
GCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGTATCCACTAGGGCCACTGGCATC6880.10383463730319713No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6860.10353279242731575No Hit
CCCAGATACCACCGAAGAAGTTGTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGTGCCAC6820.10292910267555297No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6820.10292910267555297No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA6790.1024763353617309No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6780.1023254129237902No Hit
GTGTACATGGGAGGCCAGGCGTAACCACGCTGCTGACAGTAATAAAATCC6720.10141987829614604No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6710.10126895585820535No Hit
CTCCCAGGCTCCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCA6690.10096711098232397No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGAAGAAGTTGTGTTGACACAGTCTC6650.10036342123056119No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6640.1002124987926205No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT6640.1002124987926205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)