FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662592
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25640.38696513087993817No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21560.32538877620013523No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21540.3250869313242538No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16870.25460615280595No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTAGCGG15730.23740099488071092No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15270.230458562735439No Hit
GCCTAGAGCCTGACGATTCTGGATTTTATTACTGTCAGCAGCGTGGTTAC14730.22230875108664155No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14250.21506447406548826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13880.20948034386168263No Hit
CTTTTGGCCAGGGGGCCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA13770.207820197044335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13700.2067637399787501No Hit
CTCCTGCAGGGCCAGTCAGACTTTTAGCGGCGGCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCT13120.19801023857818992No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGCCTAGAGCCTGACGATT13110.19785931614024918No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13090.19755747126436785No Hit
GTCCCAGACCCACTGCCAGCGAACCTGGCTGGGATGCCAGTGGCCCTAGT12620.19046411668115523No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12280.18533275379117164No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11960.18050323577706945No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCAGC11920.1798995460253067No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10980.16571283685888147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10910.16465637979329661No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10840.16359992272771176No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTAGCGGCGGCTTAGCCTGGTA10810.1631471554138897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10800.162996232975949No Hit
TGTATGATGTATCCACTAGGGCCACTGGCATCCCAGCCAGGTTCGCTGGCAGTGGGTCTGGGACAGACTTCAGT10680.1611851637206607No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10600.15997778421713513No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10580.15967593934125374No Hit
CTGTTGGTACCAGGCTAAGCCGCCGCTAAAAGTCTGACTGGCCCTGCAGGAGAGGGTGGCACTTTCCCCTGGAGA10530.15892132715155027No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10500.1584685598377282No Hit
CCCATGTACACTTTTGGCCAGGGGGCCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTC10270.15499734376509225No Hit
GGCCAAAAGTGTACATGGGAGGCCAGGCGTAACCACGCTGCTGACAGTAA9900.1494132135612866No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGCCCCCTGGCCAAAAGTGTACATGGGAGG9880.1491113686854052No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9850.14865860137158313No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9810.14805491161982035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9650.14564015261276925No Hit
GAAATACACAGCCGTGTCCTCGACTCTCAGGTTGCTCATGTGAAGATACA9480.14307447116777744No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9460.14277262629189608No Hit
GCTCTAGGCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCAGCGAACCTGGCTGGGATGCCAGTGG9450.14262170385395537No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9370.14141432435042983No Hit
CTCCTGTGCAGCCTCTGGATTCAGCGTCAGTGACATCTACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG9060.13673572877426832No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGCCCCCTGGCCAAAAGTG8940.13492465951898No Hit
GTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGTGC8890.13417004732927654No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8800.1328117453878103No Hit
ACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCAGCCAGGTT8760.13220805563604754No Hit
GTACATGGGAGGCCAGGCGTAACCACGCTGCTGACAGTAATAAAATCCAG8410.12692577030812324No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8390.12662392543224185No Hit
CTGCTACTCTGGCTCCCAGATACCACCGAAGAAGTTGTGTTGACACAGTC8380.12647300299430117No Hit
GTGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTAGCGGCGGCTTAGCCTGGTACCAACAGAGACCTGGCCAGG8300.12526562349077563No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7970.12028518303873273No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC7970.12028518303873273No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7950.11998333816285135No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7920.11953057084902927No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7880.1189268810972665No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7820.11802134646962234No Hit
CCTCATGTATGATGTATCCACTAGGGCCACTGGCATCCCAGCCAGGTTCGCTGGCAGTGGGTCTGGGACAGACTT7790.11756857915580027No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC7720.11651212209021541No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA7700.11621027721433401No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7700.11621027721433401No Hit
CATCATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGCCGCCGCTAAAAGTCTGA7680.11590843233845263No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7580.11439920795904569No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7550.11394644064522359No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7480.11288998357963874No Hit
GGCGTAACCACGCTGCTGACAGTAATAAAATCCAGAATCGTCAGGCTCTAGGCTGCTGATGGTGAGACTGAAGT7370.11122983676229112No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7330.11062614701052836No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA7160.10806046556553654No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7090.10700400849995169No Hit
GACTTCAGTCTCACCATCAGCAGCCTAGAGCCTGACGATTCTGGATTTTATTACTGTCAGCAGCGTGGTTACGC6990.10549478412054476No Hit
GTGTACATGGGAGGCCAGGCGTAACCACGCTGCTGACAGTAATAAAATCC6950.10489109436878198No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6900.10413648217907853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)