FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696747
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35080.5034826127704892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33900.48654676661686375No Hit
GTAATAAACCCCTACATCTTCAGCCTCCACTCTGGTGATTGTCAGTGTAAAATCCGTGCCTGATCCACGACCA27020.3878021720940313No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24930.3578056310253219No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22870.32823966231645063No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22050.31647068448088045No Hit
GATTTTACACTGACAATCACCAGAGTGGAGGCTGAAGATGTAGGGGTTTATTACTGCATGCAATCTCTACAAACT21980.3154660156412586No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20590.2955161629687677No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19200.2755663102962768No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19080.2738440208569251No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19020.27298287613724925No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18670.26795953193914No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17950.25762579530302965No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17890.2567646505833538No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGCCTAGTCAGAGCCTCCTG17730.2544682646642182No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17160.24628738982729742No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16880.24226871446881007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16500.2368147979108629No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15760.22619401303486056No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGA15600.22389762711572492No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15300.2195919035173456No Hit
CTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15290.21944837939739964No Hit
CTCCCTGCCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGCCTA15240.21873075879766976No Hit
CTACAAACTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG14850.2131333181197766No Hit
TCCGTGGAGTTTGTAGAGATTGCATGCAGTAATAAACCCCTACATCTTCA14820.2127027457599387No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14780.2121286492801548No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14700.21098045632058693No Hit
GTAATGGATACAACTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACACCTCCTGATCTATCAGGGTT14590.20940169100118122No Hit
GTCTCCACTCTCCCTGCCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCT14460.20753587744188348No Hit
GTGTAAAATCCGTGCCTGATCCACGACCACTGAACCTGTCAGGGACCCCG14330.2056700638825858No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14200.20380425032328806No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14090.20222548500388235No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13900.19949852672490873No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACTCTCC13820.19835033376534092No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCGCCC13810.19820680964539494No Hit
GAGTTTGTAGAGATTGCATGCAGTAATAAACCCCTACATCTTCAGCCTCCACTCTGGTGATTGTCAGTGTAAAAT12970.18615078356993284No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12530.17983572229230985No Hit
GGTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12110.17380770925457878No Hit
GTTCAGTGGTCGTGGATCAGGCACGGATTTTACACTGACAATCACCAGAG12010.17237246805511902No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11480.1647656896979822No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11440.1641915932181983No Hit
GTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11410.16376102085836036No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11390.1634739726184684No Hit
GACAATCACCAGAGTGGAGGCTGAAGATGTAGGGGTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTT11220.16103406257938677No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11140.15988586961981896No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACT11040.1584506284203592No Hit
GGCCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTC11040.1584506284203592No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10990.1577330078206293No Hit
ATGTAGGGGTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTC10780.15471900130176378No Hit
GTCTCCACACCTCCTGATCTATCAGGGTTCTAATCGGGCCTCCGGGGTCC10680.153283760102304No Hit
GTCGTGGATCAGGCACGGATTTTACACTGACAATCACCAGAGTGGAGGCT10520.15098737418316835No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGGCCTGCAGGAGATGGAGGCC10400.1492650847438166No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10240.146968698824681No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10220.14668165058478902No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10050.1442417405457074No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9950.14280649934624762No Hit
GTGTGGAGACTGCCCTGGCTTCTGCACGTACCAATCCAAGTAGTTGTATC9840.14122773402684188No Hit
GACTAGGCCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGCGACGGGCAGG9680.13893134810770624No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATAT9660.1386442998678143No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9600.1377831551481384No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9450.13563029334894874No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9340.134051528029543No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9140.13118104563062347No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9130.13103752151067746No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9050.12988932855110966No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9000.12917170795137975No Hit
AGCCAGGGCAGTCTCCACACCTCCTGATCTATCAGGGTTCTAATCGGGCC8820.12658827379235219No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCTGATAGATCAGGAGGTGTGGAGACTGCCCTGGCTTCTGCACGTA8780.12601417731256825No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8720.1251530325928924No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT8690.12472246023305447No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8650.12414836375327055No Hit
GTCCCTGACAGGTTCAGTGGTCGTGGATCAGGCACGGATTTTACACTGAC8620.12371779139343263No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8570.12300017079370275No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8530.12242607431391882No Hit
CAGTAATAAACCCCTACATCTTCAGCCTCCACTCTGGTGATTGTCAGTGTAAAATCCGTGCCTGATCCACGACCA8520.12228255019397286No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTC8510.12213902607402687No Hit
CCTCCTGATCTATCAGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT8390.12041673663467514No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8370.12012968839478319No Hit
CTCCAGGGGCGACGGGCAGGGAGAGTGGAGACTGAGTCAGGACGATATCC8240.11826387483548548No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8200.11768977835570156No Hit
GGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCGCCCCTGGAGAGCCGG8190.1175462542357556No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8130.11668510951607972No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8080.11596748891634984No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8070.11582396479640387No Hit
TTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTCGGCCAAGGGA8070.11582396479640387No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8020.11510634419667398No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8010.11496282007672799No Hit
CCACTCTCCCTGCCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG7990.11467577183683604No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7950.11410167535705212No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7920.11367110299721421No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7870.11295348239748432No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7830.1123793859177004No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7780.11166176531797051No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7750.11123119295813257No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7720.11080062059829465No Hit
GTAATGGATACAACTACTTGGATTGGTTCAGTGGTCGTGGATCAGGCACG7670.11008299999856477No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7630.10950890351878084No Hit
ATCCACGACCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCTGATAGATCAGGAGGTGTGGAGA7500.10764308995948313No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7420.10649489699991532No Hit
ATGCAGGAGGCTCTGACTAGGCCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGCGACGGGCAGGGAGAGTGGAGA7230.10376793872094174No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7180.10305031812121185No Hit
GGATACAACTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACACCTCCTGATCTATCAGGGTTCTAAT6970.10003631160234634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC252.3630784E-456.1301251
CCATCTA200.00774392651.08483517
CGGTACC351.8454946E-551.04837860
GGTATCA4500.049.20341
GTGGTAT1200.045.418521
TAGGTAA404.1341773E-544.53652659
TGGTATC1250.043.5892642
GTATCAA13350.043.1220781
TTAACGG350.001252934540.0160250
GTTTAAC350.001273987539.88065348
ATACGTA603.2190292E-739.7326511
AATACGT603.2190292E-739.7326510
GTATACA350.00143439638.930161
GAGTATA459.2844544E-538.8271149
GTGCTAG801.8553692E-938.3218771
AATTTCG556.941533E-637.98130847
TATCAAC15600.036.673662
CGTATGC1700.036.62237541
TCGGAGC609.136673E-636.4895466
TATACAC1700.036.0572973
Produced by FastQC (version 0.11.5)