FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696747
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35820.5141033976464915No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27320.39210789569241056No Hit
GTAATAAACCCCTACATCTTCAGCCTCCACTCTGGTGATTGTCAGTGTAAAATCCGTGCCTGATCCACGACCA25230.36211135462370125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22330.3204893598393678No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22230.31905411863990807No Hit
GATTTTACACTGACAATCACCAGAGTGGAGGCTGAAGATGTAGGGGTTTATTACTGCATGCAATCTCTACAAACT20240.29049281877065847No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19980.2867611916520631No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19750.28346013689330557No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18480.26523257366016645No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18250.261931518901409No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18140.26035275358200327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17920.25719522294319175No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGCCTAGTCAGAGCCTCCTG17820.25575998174373193No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17270.24786615514670318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17040.2445651003879457No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17030.24442157626799974No Hit
CTCCCTGCCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGCCTA16270.23351374315210544No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15470.22203181355642723No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15420.22131419295669733No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15280.21930485527745366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15220.21844371055777778No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14960.21471208343918236No Hit
CTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC14950.21456855931923638No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14900.2138509387195065No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14810.2125592216399927No Hit
TCCGTGGAGTTTGTAGAGATTGCATGCAGTAATAAACCCCTACATCTTCA14620.20983226336101912No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14350.20595711212247772No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGA14300.20523949152274784No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14140.20294310560361223No Hit
CTACAAACTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG13900.19949852672490873No Hit
GTCTCCACTCTCCCTGCCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCT13810.19820680964539494No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13690.19648452020604323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13440.19289641720739378No Hit
GAGTTTGTAGAGATTGCATGCAGTAATAAACCCCTACATCTTCAGCCTCCACTCTGGTGATTGTCAGTGTAAAAT13300.1908870795281501No Hit
GTAATGGATACAACTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACACCTCCTGATCTATCAGGGTT13230.18988241068852826No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACTCTCC13070.18758602476939262No Hit
GTGTAAAATCCGTGCCTGATCCACGACCACTGAACCTGTCAGGGACCCCG12980.1862943076898788No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCGCCC12760.18313677705106732No Hit
GTTCAGTGGTCGTGGATCAGGCACGGATTTTACACTGACAATCACCAGAG12370.17753933637317418No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11640.16706207561711783No Hit
GTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11340.16275635201873853No Hit
GGTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11310.16232577965890058No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11220.16103406257938677No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11210.16089053845944082No Hit
GACAATCACCAGAGTGGAGGCTGAAGATGTAGGGGTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTT11110.15945529725998103No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10960.15730243546079137No Hit
ATGTAGGGGTTTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTC10680.153283760102304No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10520.15098737418316835No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACT10500.1507003259432764No Hit
GTCGTGGATCAGGCACGGATTTTACACTGACAATCACCAGAGTGGAGGCT10350.14854746414408673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10310.14797336766430283No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10240.146968698824681No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10230.146825174704735No Hit
GGCCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTC10060.14438526466565338No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9860.14151478226673384No Hit
GTGTGGAGACTGCCCTGGCTTCTGCACGTACCAATCCAAGTAGTTGTATC9720.13950544458749015No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9710.13936192046754417No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9700.1392183963475982No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9520.1366349621885706No Hit
GTCTCCACACCTCCTGATCTATCAGGGTTCTAATCGGGCCTCCGGGGTCC9460.13577381746889475No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATAT9320.13376447978965103No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8980.12888465971148783No Hit
GGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCGCCCCTGGAGAGCCGG8810.1264447496724062No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8800.1263012255524602No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8770.1258706531926223No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8740.12544008083278435No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGGCCTGCAGGAGATGGAGGCC8650.12414836375327055No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8460.12142140547429699No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8400.1205602607546211No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8360.11998616427483721No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8350.11984264015489123No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8310.11926854367510732No Hit
CAGTAATAAACCCCTACATCTTCAGCCTCCACTCTGGTGATTGTCAGTGTAAAATCCGTGCCTGATCCACGACCA8280.11883797131526938No Hit
GACTAGGCCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGCGACGGGCAGG8260.11855092307537743No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATCGTCCTGACTCAGTCTC8050.1155369165565119No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8030.11524986831661994No Hit
CCTCCTGATCTATCAGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT8020.11510634419667398No Hit
CTCCAGGGGCGACGGGCAGGGAGAGTGGAGACTGAGTCAGGACGATATCC7980.11453224771689005No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7970.11438872359694409No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7930.11381462711716017No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7930.11381462711716017No Hit
TTATTACTGCATGCAATCTCTACAAACTCCACGGACGTTCGGCCAAGGGA7910.11352757887726822No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7900.11338405475732223No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7890.11324053063737627No Hit
AGCCAGGGCAGTCTCCACACCTCCTGATCTATCAGGGTTCTAATCGGGCC7760.11137471707807856No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7620.10936537939883488No Hit
GTCCCTGACAGGTTCAGTGGTCGTGGATCAGGCACGGATTTTACACTGAC7530.10807366231932107No Hit
GTAATGGATACAACTACTTGGATTGGTTCAGTGGTCGTGGATCAGGCACG7470.10721251759964522No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7260.10419851108077968No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7190.10319384224115784No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7010.10061040808213025No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6990.1003233598422383No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6970.10003631160234634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)