FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800407
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCCGCAGCAGTGACAGCATTGCCAG27450.3429505239209552No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26060.3255843589573804No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCGGTTGCTATTATCATAAGA25370.3169637446948865No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCGGTTG23830.29772353315250866No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT22320.27885813092589146No Hit
GTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCCGCAGCAGTGACAGCATTGCCAGCAGCTATGTG21660.2706123259791581No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT20240.2528713516998227No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19800.24737414840200048No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19260.24062758071830959No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18500.23113241138570753No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17520.21888864040419437No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGG17430.21776421245691255No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16490.20602018722974688No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15860.19814919159877412No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA15490.19352654337105998No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15340.19165249679225693No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15070.18827921295041147No Hit
GACTACTACTGTCAGTCTTATGATAATAGCAACCGCAATTGGGTGTTCGG14950.18677997568736907No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGAT14350.17928378937215692No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13950.17428633182868214No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG13940.17416139539009529No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13760.17191253949553165No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAAC13670.17078811154824983No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13490.1685392556536862No Hit
GTGTATTACTGTGCGAGAGGTTCGACGATTTCGCGAGATTTTTGGAGTGC13070.16329192523303768No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGC12880.16091813289988718No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCGGTTGC12640.15791965837380234No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12420.1551710567248912No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12400.15492118384771747No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACG12170.15204764576021948No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12120.15142296356728513No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12120.15142296356728513No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11700.14617563314663665No Hit
CTACTACTGTCAGTCTTATGATAATAGCAACCGCAATTGGGTGTTCGGCG11590.1448013323221811No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11410.14255247642761745No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11120.13892931970859826No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10680.133432116410776No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10570.13205781558632046No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAA10340.12918427749882247No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA10210.12756010379719318No Hit
GCTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTTTGAGGATTACCAAAGACCCT10190.12731023092001945No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA10120.12643567584991136No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT9980.12468656570969519No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9860.12318732844665277No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9770.12206290049937094No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9710.12131328186784975No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9660.1206885996749154No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTTTGAGGATTACCAAAGACCCTCTGGGGTCC9370.11706544295589619No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9330.11656569720154872No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9260.11569114213144063No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9220.11519139637709316No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9150.11431684130698506No Hit
GTCTTATGATAATAGCAACCGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8950.11181811253524769No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8910.11131836678090021No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8870.11081862102655274No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8830.11031887527220526No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8820.11019393883361839No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8720.10894457444774971No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGA8690.10856976513198911No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8580.10719546430753353No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT8490.10607103636025172No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8470.10582116348307799No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8380.10469673553579616No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC8360.10444686265862244No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8310.10382218046568809No Hit
GTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTT8230.10282268895699313No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8200.10244787964123253No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC8160.10194813388688506No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8070.10082370593960323No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8030.10032396018525576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3850.066.5524170
CCCTAGT200.007544028651.42456435
GGTATCA6150.045.373591
TACGTAT608.0292625E-443.79944270
CGTATGC3550.042.8586941
TCGTATG3650.042.5399940
ATGCCGT3700.042.23816344
CTTATAC3650.041.9550251
TCTCGTA3550.041.69132638
ATCTCGT3500.041.25395237
CACGAGA4100.039.83026521
CCGTCTT3800.039.46492847
CGCGGTA6700.039.22338570
TATACAC4100.038.990723
AGAGCGT350.001445065438.8722083
TTATACA4050.038.6322562
ATCTCCG4150.038.51854710
GTATGCC4050.038.4603842
CCCACGA4200.038.0623719
CGAGACA4250.037.63103523
Produced by FastQC (version 0.11.5)