FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800407
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40900.510990033820294No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25730.32146145648401375No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCGGTTGCTATTATCATAAGA24880.31084185920412993No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCCGCAGCAGTGACAGCATTGCCAG24370.3044701008361996No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT24070.30072200767859353No Hit
GTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCCGCAGCAGTGACAGCATTGCCAGCAGCTATGTG21710.27123700817209245No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCGGTTG20570.2569942541731894No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20300.25362097033134395No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18720.23388101303461864No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18110.22625989028081964No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGG16600.2073944880542024No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA16290.20352145845800948No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16010.20002323817757714No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15660.19565046282703674No Hit
GACTACTACTGTCAGTCTTATGATAATAGCAACCGCAATTGGGTGTTCGG15360.19190236966943067No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15310.19127768747649632No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAAC15250.19052806884497514No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG14210.17753467923194075No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14190.177284806354767No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14090.1760354419688983No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13870.1732868403199872No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13410.16753976414499125No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGC13400.16741482770640437No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGAT13220.16516597181184073No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12700.15866927700532354No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12300.1536718194618488No Hit
GTGTATTACTGTGCGAGAGGTTCGACGATTTCGCGAGATTTTTGGAGTGC12280.15342194658467503No Hit
CTACTACTGTCAGTCTTATGATAATAGCAACCGCAATTGGGTGTTCGGCG11990.14979878986565587No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11930.14904917123413464No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11460.1431771586205518No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACG11140.13917919258577197No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11040.1379298281999033No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10840.13543109942816592No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10830.13530616298957904No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10810.13505629011240533No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA10580.1321827520249073No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10240.1279349131129538No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCGGTTGC10140.1266855487270851No Hit
GCTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTTTGAGGATTACCAAAGACCCT9880.12343720132382649No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9830.12281251913089217No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTTTGAGGATTACCAAAGACCCTCTGGGGTCC9770.12206290049937094No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAA9600.11993898104339416No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9520.11893948953469921No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9010.11256773116676891No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9010.11256773116676891No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8950.11181811253524769No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8860.11069368458796586No Hit
GTCTTATGATAATAGCAACCGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8810.11006900239503153No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8690.10856976513198911No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGA8620.10769521006188101No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGATAGCATAGTTGCTGAAGGTGCCTCCAGAAGCCTTGCAGGAGACCTTCA8580.10719546430753353No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8570.10707052786894668No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8290.10357230758851434No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8290.10357230758851434No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACC8270.1033224347113406No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTTTGAGGAT8240.10294762539558No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8170.1020730703254719No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8140.1016982610097113No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8120.10144838813253758No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8120.10144838813253758No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC8010.10007408730808201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAT200.007812442750.97181720
GTGGTAT2550.047.9854771
CGGGATT405.4723176E-542.47120317
TGGTATC2950.041.4375042
GGTATCA6550.036.843811
TACACCG502.0438993E-433.9536135
CGCGGTA6500.033.0248170
TATCAAC19900.032.761312
GTATCAA20400.032.6567841
ATAGCGG450.003706561632.08095663
TTGCCCC553.576929E-430.8649923
ATCAACG21550.030.091583
GACAGGA1151.9954314E-929.5562731
TCAACGC22050.029.409234
CAACGCA22200.029.2123435
AACGCAG22300.029.0831626
AATGGTA3500.028.74927770
ACGCAGA22950.028.2594597
CGCAGAG23200.028.1012978
AGAGTAC23850.027.6201811
Produced by FastQC (version 0.11.5)