FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501170
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22710.45313965321148514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21630.4315900792146377No Hit
ATTAGGAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAC18300.365145559391025No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17180.342797853023924No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT17020.3396053235429096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15860.316459484805555No Hit
GAGTTCTCTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTTTGCCAACAGTATCATAATTAT15680.31286788913941377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15680.31286788913941377No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15440.3080790949178921No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15430.3078795618253287No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15390.3070814294550751No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14780.29490991080870765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14280.2849332561805375No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14030.2799449288664525No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCTCTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG13190.26318414909112675No Hit
GTATCATAATTATCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT13110.2615878843506195No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12550.25041403116706906No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12450.24841870024143506No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGGAACTGGTTGGCCTGGTATCAGCAGAAA12210.2436299060199134No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12070.2408364427240258No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11610.23165792046610933No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11130.222080332023066No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10970.2188878025420516No Hit
TTATGATACTGTTGGCAAAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGAGAACTCTGTC10930.218089670171798No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10690.21330087595027636No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10670.21290180976514958No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10390.2073148831733743No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10300.20551908534030366No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10050.20053075802621864No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10030.20013169184109184No Hit
CTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCATCAAGGTT9800.19554243071213362No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9630.19215036813855577No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9470.18895783865754134No Hit
ACCTTGATGAGACCCCACTTTCTAAACTAGACGCCTTATAGATTAGGAGGGTAGGGGCTTTCCCTGGTTTCTG9080.1811760480475687No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC8930.17818305165911766No Hit
GATTAGGAGGGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC8690.173394257437596No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8680.17319472434503264No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8560.1708003272342718No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8450.1686054632160744No Hit
GGGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTCCTA8340.16641059919787696No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCTCT8290.16541293373505994No Hit
GTTCCTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA8230.16421573517967955No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8200.16361713590198934No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8060.16082367260610173No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7750.15463814673663628No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7690.15344094818125587No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7540.15044795179280482No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7390.14745495540435383No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7290.1454596244787198No Hit
CCTTATAGATTAGGAGGGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTCCTAATAGTCTGA7270.145060558293593No Hit
GACTATTAGGAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTACCCTCCTAATCTATAAGGCGT7250.14466149210846618No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7190.14346429355308576No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7190.14346429355308576No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC7080.14126942953488836No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6980.13927409860925435No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGGAA6980.13927409860925435No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6900.13767783386874713No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6890.13747830077618373No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6880.13727876768362032No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6870.13707923459105692No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6840.1364806353133667No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6830.1362811022208033No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6780.13528343675798632No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6760.1348843705728595No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6750.1346848374802961No Hit
ATCTGGGACAGAGTTCTCTCTCACCATCAGCAGCCTGCAGCCTGATGATT6700.1336871720174791No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6590.1314923079992817No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6570.13109324181415488No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6530.13029510944390127No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6470.12909791088852085No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6260.12490771594468941No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6080.12131612027854818No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6030.12031845481573118No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5930.11832312389009718No Hit
CAGTATCATAATTATCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5910.11792405770497036No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5910.11792405770497036No Hit
CCTTGGTCCCTTGGCCGAACGTCGGATAATTATGATACTGTTGGCAAAAA5830.11632779296446316No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTACCCTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCAT5810.11592872677933636No Hit
GCCCCTACCCTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGAT5790.11552966059420955No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5640.11253666420575853No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC5530.11034180018756111No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTTTTGCCAACAGTATCATAAT5510.10994273400243432No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA5500.1097432009098709No Hit
CCCCTACCCTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCA5480.1093441347247441No Hit
TAATTATCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT5430.10834646926192709No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5420.10814693616936369No Hit
AGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCTCTCTCACCATCAGCAGC5390.10754833689167348No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5310.10595207215116627No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG5310.10595207215116627No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5300.10575253905860287No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5280.10535347287347607No Hit
CTATTAGGAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT5250.10475487359578586No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC5030.10036514555939102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAG200.0051654956.58783764
GACGGAC253.0873687E-453.1698657
ATATAAG200.0085075849.881591
CGGGATT352.8291579E-547.4873117
CTTGAAA3750.041.06165357
TCGTATG3700.040.19191740
GTATCAA22050.039.9655951
CGTATGC3750.039.82971641
ATCTCGT3700.039.7666337
GACGGAG3650.038.41490626
GCCGTCT3900.038.05402446
CTTATAC3700.037.748231
ATTACCG400.001707128737.57202563
GTATGCC4100.037.4055542
CCGTCTT4100.037.1502347
TTATACA3850.037.1153342
TATACAC3950.037.0173
GGAGCTA3900.036.9677529
CCCACGA3900.036.6541219
TCTCCGA3900.036.63949611
Produced by FastQC (version 0.11.5)