FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501170
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32050.6395035616657022No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22430.4475527266197099No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19210.38330307081429454No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17350.34618991559750184No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16470.3286310034519225No Hit
ATTAGGAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAC16460.3284314703593591No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14990.2991001057525391No Hit
GAGTTCTCTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTTTGCCAACAGTATCATAATTAT14900.29730430791946844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14790.29510944390127103No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14320.28573138855079117No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13380.2669752778498314No Hit
GTATCATAATTATCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT13260.2645808807390706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13030.25999161961011236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12920.25779675559191495No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12860.2565995570365345No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCTCTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG12120.24183410818684278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11990.23924017798351857No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11560.2306602550032923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11160.22267893130075625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10960.2186882694494882No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10860.21669293852385418No Hit
TTATGATACTGTTGGCAAAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGAGAACTCTGTC10670.21290180976514958No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10460.20871161482131811No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10390.2073148831733743No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10380.2071153500808109No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10090.20132889039647228No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10080.20112935730390882No Hit
CTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCATCAAGGTT9860.19673962926751404No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGGAACTGGTTGGCCTGGTATCAGCAGAAA9730.1941456990641898No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9330.18616437536165373No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9330.18616437536165373No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9160.1827723127880759No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9000.17957978330706148No Hit
ACCTTGATGAGACCCCACTTTCTAAACTAGACGCCTTATAGATTAGGAGGGTAGGGGCTTTCCCTGGTTTCTG8670.17299519125246923No Hit
GGGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTCCTA8350.16661013229044036No Hit
GTTCCTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA8120.16202087116148212No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8090.16142227188379193No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCTCT8010.1598260071432847No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8010.1598260071432847No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8000.1596264740507213No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7850.1566334776622703No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC7700.15364048127381927No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7660.15284234890356566No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7650.15264281581100225No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGAGACCCCACTTTCTAAACT7620.15204421653331204No Hit
GATTAGGAGGGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC7530.15024841870024144No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7480.14925075323742443No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7460.14885168705229762No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7290.1454596244787198No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7290.1454596244787198No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7230.14426242592333938No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC7040.14047129716463477No Hit
CCTTATAGATTAGGAGGGTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTCCTAATAGTCTGA6990.13947363170181776No Hit
GAGAGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGAGACCCCA6860.13687970149849354No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6830.1362811022208033No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6670.13308857273978889No Hit
GACTATTAGGAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTACCCTCCTAATCTATAAGGCGT6590.1314923079992817No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6590.1314923079992817No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6560.13089370872159148No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6530.13029510944390127No Hit
ATCTGGGACAGAGTTCTCTCTCACCATCAGCAGCCTGCAGCCTGATGATT6510.12989604325877446No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6500.12969651016621106No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6460.12889837779595748No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6350.12670351377776004No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6320.12610491450006983No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6320.12610491450006983No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGGAA6320.12610491450006983No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6150.12271285192649199No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6130.1223137857413652No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6010.11991938863060439No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5880.11732545842728016No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5830.11632779296446316No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5810.11592872677933636No Hit
CAGTATCATAATTATCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5770.11513059440908274No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5770.11513059440908274No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5760.11493106131651935No Hit
CCTTGGTCCCTTGGCCGAACGTCGGATAATTATGATACTGTTGGCAAAAA5750.11473152822395595No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5730.11433246203882913No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5720.11413292894626574No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTACCCTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCAT5620.11213759802063171No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5570.11113993255781472No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG5550.1107408663726879No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5400.1077478699842369No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC5350.10675020452141988No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5290.10555300596603948No Hit
TAATTATCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT5280.10535347287347607No Hit
GCCCCTACCCTCCTAATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGAT5270.10515393978091266No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTTTTGCCAACAGTATCATAAT5170.10315860885527865No Hit
AGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCTCTCTCACCATCAGCAGC5150.10275954267015183No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5030.10036514555939102No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5020.10016561246682762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG201.0200478E-466.519617
AAATACG201.02055186E-466.512975
TAGGACG305.572792E-447.1707247
AATGGTA2250.041.60170470
TTTAGGG350.001532781338.40595630
AATACGG350.001612608838.0112046
AGGACGA400.002262958635.47594548
ATCAGAC400.002866612733.8063232
TAGGGGA400.002866612733.8063232
TTAGGGG400.002912517433.69694531
GTATCAA22500.032.694621
GGACGAT450.00397200131.62843949
AGGAGTC1101.5352271E-930.2845421
ACCGTGC450.005525946629.5642729
GCAGAGT25700.028.7302599
AACGCAG25550.028.5084046
AGAGTAC25800.028.48998811
TATCAAC25600.028.3228022
CGCAGAG25900.028.2515728
CTAGGAC500.006916240328.23649446
Produced by FastQC (version 0.11.5)