FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402188
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGAGTCCTCGACTCTCAGGCTGCTCATTTCCAGATATA49730.3546600027956308No Hit
ACCATATACTACGCAGACTCTGTTAAGGGCCGATTTATCATCTCCAAAGA44750.31914408053698934No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42220.301100850955792No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39380.2808467908725506No Hit
GATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA34930.2491106756012746No Hit
GTATATGGTAGAACCACTTTTACTGATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT34060.2429060867729577No Hit
GTTTATTACTGTGCGAGAGACTGGGTGCAGTGGCTGGTCCCCGATGCTTT32330.23056822622929307No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30230.21559163250576954No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28850.20574987091602553No Hit
GACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28640.20425221154367315No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28180.20097162434709182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26900.1918430338870394No Hit
CTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAATCTGTC25600.18257180920104865No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25050.17864936798774486No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24790.1767951230505467No Hit
GTCCTGATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTT24240.17287268183724294No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23580.1681657523812784No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23530.1678091668164326No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT23120.16488516518469706No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22670.16167589510108488No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22470.16024955284170167No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATG22360.15946506459904095No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21900.15618447740245958No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21300.15190545062431002No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21120.15062174259086514No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20920.14919540033148193No Hit
ATCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTC20490.14612876447380807No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20480.14605744736083892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20480.14605744736083892No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19770.14099393234002858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19750.14085129811409028No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19410.13842651627313884No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19400.1383551991601697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19190.1368575397878173No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19140.13650095422297154No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT19080.13607305154515656No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19030.13571646598031076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18970.1352885633024958No Hit
CTCCTGTGCAGCCTCTGGATTCAGCCTCAATAGTTATGAAATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG18790.13400485526905093No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18700.1333630012523285No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC18070.12887002313527143No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18000.12837080334448733No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC17480.12466231347009102No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17310.12344992254961533No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTAGTAGTTATTTACATTGGTATCAGAAGAAA17260.12309333698476953No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATGTAAAT17210.12273675141992373No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17140.1222375316291396No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16950.12088250648272557No Hit
ACTTACTACTGTCAACAGACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16520.1178158706250517No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16490.11760191928614422No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16420.1171026994953601No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16280.11610425991379186No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16230.11574767434894608No Hit
GATTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTATAGAATC16050.11446396631550121No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15930.11360816095987128No Hit
GTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGC15920.11353684384690212No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15900.1133942096209638No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15900.1133942096209638No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15870.11318025828205631No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15870.11318025828205631No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15790.11260972137830305No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15240.10868728016499928No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15110.10776015769640021No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGA15080.10754620635749271No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14660.10455088761278802No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14600.10412298493497306No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14590.1040516678220039No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTTGATGGGACCCCA14360.10241137422371323No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14280.10184083731995994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4350.046.0751541
TCGTATG4300.045.77250740
CTTATAC4550.044.2066461
GCCGTCT4850.042.35469446
CCCACGA4700.042.0569419
ATCTCGT4350.041.9001137
TATACAC4900.041.7254263
ACGAGAC4850.041.46629322
TTATACA5050.041.1608242
ATGCCGT4950.040.65642544
CACGAGA5050.040.49616221
GTATGCC5100.040.0232842
CCGTCTT5150.039.26717447
TTTGGAG16950.039.16454770
CTACATC4650.039.0311233
GTGGTAT2900.038.7938541
TCTCCGA5100.038.75144611
ATCTCCG5250.037.6442610
TACACAT5350.037.5788765
CATCTCG4850.037.5318836
Produced by FastQC (version 0.11.5)