FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402188
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47260.3370446758922484No Hit
GTAATAAACAGCCGAGTCCTCGACTCTCAGGCTGCTCATTTCCAGATATA45920.327488182754381No Hit
ACCATATACTACGCAGACTCTGTTAAGGGCCGATTTATCATCTCCAAAGA45340.32335179020216975No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42960.30637831731550974No Hit
GTTTATTACTGTGCGAGAGACTGGGTGCAGTGGCTGGTCCCCGATGCTTT33350.23784257175214737No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33110.23613096104088752No Hit
GATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA32800.23392013053884358No Hit
GTATATGGTAGAACCACTTTTACTGATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT31730.2262891994511435No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29820.21266763087403404No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27560.19654996334300393No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27010.19262752212970016No Hit
GACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26880.19170039966110108No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24970.17807883108399158No Hit
GTCCTGATCTATGCTGCACACATTTTGCAAAGTGGGGTCCCATCAAGGTT24540.17501219522631772No Hit
CTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAATCTGTC23880.17030526577035318No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23030.1642433111679746No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23000.16402935982906713No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22440.1600356015027942No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATG21150.1508356939297726No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21080.15033647413898849No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20950.14940935167038943No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20360.14520164200520902No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19920.14206368903456598No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19900.14192105480862766No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19560.13949627296767625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19380.13821256493423134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19230.13714280823969396No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19120.1363583199970332No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTAGTAGTTATTTACATTGGTATCAGAAGAAA19050.13585910020624908No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18940.13507461196358833No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18880.13464670928577338No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18830.13429012372092758No Hit
CTCCTGTGCAGCCTCTGGATTCAGCCTCAATAGTTATGAAATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG18810.13414748949498928No Hit
ATCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTC18770.1338622210431126No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT18440.13150875631513034No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18350.13086690229840792No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18190.12972582849090136No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17740.12651655840728918No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17640.12580338727759757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17510.12487626480899852No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17480.12466231347009102No Hit
GTGCTGGGTTTTCCTTGTTACTATTTTTGAAGGTGTCCAGTGTGAGGTGC17470.12459099635712186No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17250.12302201987180036No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16930.12073987225678724No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16660.11881431020661995No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATGTAAAT16660.11881431020661995No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC16660.11881431020661995No Hit
ACTTACTACTGTCAACAGACTTATAGAATCCCTCAGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16310.11631821125269937No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16160.11524845455816196No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16020.11425001497659372No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC15960.11382211229877877No Hit
GATTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTATAGAATC15820.11282367271721053No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15510.1106128422151666No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15250.10875859727796844No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15190.10833069460015347No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15160.108116743261246No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15060.10740357213155441No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14800.10554932719435625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14730.10505010740357212No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14710.10490747317763383No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGA14500.10340981380528147No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTTGATGGGACCCCA14420.10283927690152818No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14200.10127030041620667No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14130.10077108062542255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA200.007765422651.051731
GTGGTAT3200.044.670261
TACGTCG350.001448088138.857715
TATCTGG17100.038.56904670
TTTGGAG16600.037.80208670
GTATCAA29050.037.7249761
TATCAAC31400.034.5468372
TGATCCG400.00278899334.00055
ATCAACG33450.032.5231553
TCAACGC33800.031.9852144
CAACGCA34250.031.5683465
CGATCGT450.00420504231.26636752
AACGCAG34600.031.2534716
ACAATGG14050.030.53488370
CGCAGAG37150.029.1997498
AGAGTAC37850.028.65972911
GCAGAGT38700.027.9423799
AATACGG500.00806738127.3633732
GGGCGTA500.00831028227.1974893
CTTTGGA17200.026.65920469
Produced by FastQC (version 0.11.5)