FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271491
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52610.4137662004685837No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48870.3843519144060005No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG46720.3674426323112No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35230.27707628288363817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33750.2654364049765197No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30940.2433363665177339No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28760.22619114095184314No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28720.22587654965705617No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA28500.22414629753572773No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28450.22375305841724402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28280.2224160454143993No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27790.21856230205325872No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27500.21628151516605307No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26970.21211318051012554No Hit
ATATTACTGTCAACACTATGATAATCTCCCTTTATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAA26920.21171994139164177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26030.20472028508263132No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA25140.1977206287736209No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT24360.19158609852527464No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22640.17805867284943425No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA22260.17507005554895788No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21580.1697220035375791No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21570.16964335571388237No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21420.16846363835843117No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21250.16712662535558648No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20980.1650031341157743No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20560.16169992552051096No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA20470.16099209510724025No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20330.1598910255754858No Hit
CCCTTTATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTT20140.15839671692524762No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT19770.15548674744846797No Hit
ACATATTACTGTCAACACTATGATAATCTCCCTTTATTCCCTTTCGGCCC19660.15462162138780378No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19390.1524981301479916No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC19160.15068923020296643No Hit
GAATAAAGGGAGATTATCATAGTGTTGACAGTAATATGTTGCAATATCTT19080.15006004761339248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATATCCACTTTGGTCC18950.14903762590533476No Hit
GTGCAGCCACAGTTCGTTGGATATCCACTTTGGTCCCAGGGCCGAAAGGG18440.14502658689680067No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18430.14494793907310394No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA18230.143374982599169No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17630.1386561131773642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17480.137476395821913No Hit
CTATGATAATCTCCCTTTATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTGTGGCTGCACCAT17320.13621803064276508No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17130.13472372199252688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16950.13330806116598545No Hit
GTAAAATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16790.13204969598683752No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16760.13181375251574726No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16600.1305553873365993No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT15960.12552192662000752No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG15960.12552192662000752No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15960.12552192662000752No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15850.12465680055934332No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15800.12426356144085958No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15690.12339843538019538No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC15500.1219041267299572No Hit
ATGATAATCTCCCTTTATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTGTGGCTGCACCAT15320.12048846590341576No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15030.11820767901621011No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15000.11797173554511987No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14840.11671337036597192No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA14810.11647742689488166No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC14760.11608418777639795No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14730.1158482443053077No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14590.11474717477355326No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14510.11411799218397928No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14470.1138034008891923No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACACTATGATAATCTC14450.1136461052417988No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT14370.11301692265222484No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG14330.11270233135743785No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA14280.1123090922389541No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13920.10947777058587123No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC13860.10900588364369075No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG13850.108927235819994No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13800.10853399670151029No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC13730.10798346193563306No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13680.1075902228171493No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13680.1075902228171493No Hit
GTGGATATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC13680.1075902228171493No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13560.10664644893278837No Hit
CTCCTGTGCAGCCACTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCTGGGAAGGGG13460.10585997069582088No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13350.10499484463515668No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13250.10420836639818921No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13190.10373647945600872No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13140.103343240337525No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13000.10224217080577055No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12980.10208487515837705No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12980.10208487515837705No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12890.10137704474510634No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACACTATGATAATCTCCCTT12890.10137704474510634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG200.007786876551.0158426
ACGGATC200.007788087251.0138328
CGTATGC4850.045.55714441
TCGTATG4700.045.4875240
GTAATAC306.772073E-445.3474123
ATGCCGT5000.044.3746644
ACTAGCG4850.044.2622227
CTTATAC5150.043.605211
CTAGCGC4950.043.38683728
AGACTAG5050.043.15340425
GTCGTAT405.052826E-543.051639
TATACAC5150.042.925953
ATCTCGT4600.042.55352437
CGAGACT5200.042.54331223
GACTAGC5050.042.4941926
TAGCGCT5100.042.1290229
ACGAGAC5250.042.12487822
TTATACA5250.042.108312
GAGACTA5300.041.7471824
TCTCCGA5300.041.70942311
Produced by FastQC (version 0.11.5)