FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547121
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22640.4138024312720586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22440.4101469327625881No Hit
GAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT19070.34855178287801053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18140.3315537148089728No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17580.32131831898245544No Hit
TTATAATACTTATTCTCATGTGTACACTTTTGGCCAGGGGACCGAGCTGG15350.2805595106018596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13710.2505844228242016No Hit
CTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGGCTGCACCA13110.23961792729579015No Hit
GTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC12830.23450022938253148No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12580.22993085624569337No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12210.22316818400317295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12130.2217059845993848No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11560.21128781384739392No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11560.21128781384739392No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11380.20799786518887045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11070.20233184249919123No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10190.1862476490575211No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9680.17692612785837136No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9460.17290507949795383No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9390.17162565501963917No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9360.17107733024321858No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8980.1641318830752247No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8780.1604763845657542No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8750.1599280597893336No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA8730.15956250993838658No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8610.15736921083270428No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8190.14969266396281627No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7900.14439219112408408No Hit
GTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT7720.14110224246556063No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAA7610.13909171828535188No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA7610.13909171828535188No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7490.13689841917966958No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7490.13689841917966958No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC7480.13671564425419605No Hit
TGTGTACACTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGG7120.1301357469371492No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7110.12995297201167566No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC6960.1272113481295728No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT6950.12702857320409927No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6920.12648024842767872No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA6860.12538359887483755No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6830.12483527409841699No Hit
GGCCAAAAGTGTACACATGAGAATAAGTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGG6760.12355584962010231No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6540.11953480125968477No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG6200.11332045379358496No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTAATACTCTGA6140.11222380424074382No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6100.11149270453884971No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCCCCTGGCCAAAAGTG6080.11112715468790267No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT6080.11112715468790267No Hit
CTAGTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGAAAGGGTGGAAGGAGA5970.10911663050769392No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCC5940.10856830573127334No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5930.10838553080579981No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG5880.10747165617843218No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5870.10728888125295867No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5860.10710610632748514No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5850.10692333140201163No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5820.10637500662559105No Hit
CCTTCCACCCTTTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG5780.10564390692369696No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5760.10527835707274989No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTA5740.10491280722180285No Hit
ATTCTCATGTGTACACTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGA5710.10436448244538227No Hit
ACTTATTACTGCCAACATTATAATACTTATTCTCATGTGTACACTTTTGGCCAGGGGACCGAGCTGGAGATCAAA5550.10144008363780589No Hit
CTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT5520.10089175886138532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACT252.7988857E-454.237578
GGGACGT200.007597213651.3310533
CGATGCA350.001464890838.76238319
GTATCAA18200.036.885311
ACGGGAT656.230439E-736.51941316
GTGGTAT2100.035.519191
TATCAAC21150.031.4055822
TGTTAGA7700.031.01550770
ATCAACG21550.030.822653
TCAACGC21850.030.3994584
AACGCAG22150.029.995956
CAACGCA22200.029.922925
GCCGTTA704.332416E-529.06115515
ACGCAGA22950.028.9503387
CGCAGAG23200.028.9306018
AGAGTAC23400.028.53846511
GCAGAGT23750.028.2606289
CCGTTAA605.9929525E-428.2590716
GCTAAAC500.00840165327.13366919
GTTACTC500.00843544727.1113513
Produced by FastQC (version 0.11.5)