FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009192
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG43500.43103789962663197No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCAACTGATGGCCAGGGAGG40150.39784302689676493No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC29710.2943939309863733No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28970.2870613322341041No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA27960.27705332582898No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27530.2727924914188777No Hit
GTATATGGTGGTACTACTACCAGTAATGTATGAAACCCACTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGTT26680.26436991177100094No Hit
ACCATATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAAAACTCACTGTATCTG25590.25356919198725314No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24210.23989488620599447No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23870.2365258543468438No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23860.23642676517451586No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23590.2337513575216609No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22160.21958160587876244No Hit
ATAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA20050.19867379051756257No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19490.19312479686719675No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19480.19302570769486876No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19440.19262935100555692No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGG19300.19124210259296545No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19000.18826942742312663No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGC18960.1878730707338148No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC17310.17152335729970114No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGCTGTCATCCCATG17260.17102791143806134No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17020.16864977130219028No Hit
CAATAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA16370.1622089751008728No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16360.16210988592854483No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15990.15844358655241025No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15710.15566908972722732No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15500.1535882171083401No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15290.15150734448945294No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14960.14823740180263023No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14800.14665197504538283No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14720.14585926166675914No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14510.14377838904787196No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14030.1390221087761298No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG14000.13872484125914591No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14000.13872484125914591No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13850.13723850367422652No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13800.1367430578125867No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13750.1362476119509469No Hit
GTGTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAA13570.1344640068490436No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTTGGCTCCAGT13560.13436491767671563No Hit
GCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGACCAAGGT13140.13020317243894125No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13100.1298068157496294No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13010.12891501319867776No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12970.12851865650936592No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTCGTACTTTTAGTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGG12900.12782503230307019No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGACTTGGGGCTGTGCTGGG12850.12732958644143036No Hit
GTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAAGG12790.1267350514074626No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12780.12663596223513462No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTTTA12490.12376237623762376No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12480.12366328706529581No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12330.12217694948037638No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGAC12300.12187968196339251No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12270.12158241444640862No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12150.12039334437847307No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11830.11722249086397832No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC11780.1167270450023385No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11670.11563706410673094No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG11600.11494343990043521No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11560.11454708321112336No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11430.11325892397085985No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11410.11306074562620393No Hit
CTCCAGAGACAATGCCAAAAACTCACTGTATCTGCAAATGAACAGCCTGA11400.11296165645387597No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11220.11117805135197267No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11160.1105835163180049No Hit
GGCAGAGGGTGACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTTATACTGTAAACTGGTACCAGCAA11100.10998898128403714No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTTGGCTCCAGTCTGAGGATG11050.10949353542239733No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGACCATCTCTTGTTCTGG11030.1092953570777414No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11000.10899808956075753No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10960.10860173287144569No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10950.10850264369911772No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10790.10691721694187031No Hit
TCCTCAGACTGGAGCCAACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10720.10622359273557459No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10660.10562905770160683No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10650.10552996852927887No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10540.1044399876336713No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10540.1044399876336713No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10400.10305273922107984No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10310.10216093667012818No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC10240.10146731246383246No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCCTTG10220.10126913411917651No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10190.10097186660219264No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10140.10047642074055282No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10120.1002782423958969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)