FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009192
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG41910.41528272122648613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37800.374557071399694No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCAACTGATGGCCAGGGAGG37090.36752174016440875No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28360.28101689272209845No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC28160.2790351092755392No Hit
ACCATATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAAAACTCACTGTATCTG26620.26377537673703316No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26590.26347810922004933No Hit
GTATATGGTGGTACTACTACCAGTAATGTATGAAACCCACTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGTT25890.25654186715709204No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA25830.25594733212312426No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23630.23414771421097275No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22430.22225701353161736No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21510.21314080967744492No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21000.2080872618887189No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19640.19461113445211614No Hit
ATAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA18850.1867830898382072No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18530.18361223632371243No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17680.17518965667583572No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGG17520.17360422991858834No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGC17500.17340605157393243No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC17190.1703342872317656No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16500.16349713434113627No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGCTGTCATCCCATG16500.16349713434113627No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16080.1593353891033619No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15580.15438093048696383No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15280.15140825531712498No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14740.14605744001141507No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14730.1459583508390871No Hit
GTGTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAA14660.14526472663279139No Hit
CAATAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA14530.14397656739252787No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14480.14348112153088807No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC14040.13912119794845776No Hit
GTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAAGG13960.13832848456983407No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13910.13783303870819427No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTCGTACTTTTAGTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGG13820.13694123615724263No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13660.13535580939999525No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13550.13426582850438767No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13510.1338694718150758No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13460.133374025953436No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13270.13149133167920474No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGAC13170.13050043995592514No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13040.12921228071566165No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12900.12782503230307019No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12890.12772594313074223No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12800.12683414057979056No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12500.12386146540995173No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTTGGCTCCAGT12480.12366328706529581No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12460.12346510872063989No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG12120.12009607686148918No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12050.11940245265519346No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC12010.11900609596588162No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTTTA11890.11781702589794608No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11510.11405163734948354No Hit
GCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGACCAAGGT11490.11385345900482761No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11380.11276347810922005No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGACTTGGGGCTGTGCTGGG11240.1113762296966286No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11140.11038533797334898No Hit
TCCTCAGACTGGAGCCAACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC11030.1092953570777414No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11020.10919626790541345No Hit
CTCCAGAGACAATGCCAAAAACTCACTGTATCTGCAAATGAACAGCCTGA10950.10850264369911772No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10860.10761084114816605No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10760.10661994942488644No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10700.10602541439091867No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10590.10493543349531109No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTTGGCTCCAGTCTGAGGATG10580.10483634432298314No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10490.1039445417720315No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10450.10354818508271965No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGACCATCTCTTGTTCTGG10400.10305273922107984No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10360.102656382531768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)