FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517305
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATAGGGCCGTGTCCTCGGCTCTCAGGGAGTTCATTTGCAGATACA46370.3056076398614649No Hit
GTATGGTACTTCACCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT45710.3012578222572258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44260.29170140479336715No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42080.2773338254339108No Hit
GTACCATACTGCTGACAGGAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC31110.20503458434527008No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30300.19969617183097665No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29980.19758716935619403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29530.194621384626031No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29210.19251238215124844No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28240.1861194683995637No Hit
CTATATCACTGTGCGAGATCTCTTGTTGTGGCCATACGTCCTACTACCGA27120.17873795973782464No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26650.1756403623529877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26640.17557445602565075No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT26630.17550854969831378No Hit
GCCCTATATCACTGTGCGAGATCTCTTGTTGTGGCCATACGTCCTACTAC26240.1729382029321725No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25930.17089510678472686No Hit
GTATTCCTGTCAGCAGTATGGTACTTCACCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAA25490.16799522838190079No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24700.1627886285222813No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGAATTCACCTTTAACAACTATGCC24110.15890015520940087No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23990.1581092792813574No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23900.1575161223353248No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23870.15731840335331393No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23790.1567911527346183No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23380.1540889933138031No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23300.15356174269510744No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23220.15303449207641182No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22580.1488164871268466No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22480.14815742385347705No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAGGTCCTTAGCCTGGTATC22400.14763017323478142No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22390.14756426690744445No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22280.14683929730673792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22080.14552117075999882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21200.13972141395434667No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20970.1382055684255967No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20880.1376124114795641No Hit
GTGTATTCCTGTCAGCAGTATGGTACTTCACCTCCGGTCACCTTCGGCCA20860.13748059882489017No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20840.13734878617021629No Hit
GTAGTAGGACGTATGGCCACAACAAGAGATCTCGCACAGTGATATAGGGC20700.13642609758749888No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20410.13451481409472715No Hit
TCCTACTACCGATGCTTTTGCTGTCTGGGGCCAAGGGACAGTGGTCACCGTCTCTTCAGCATCCCCGACCAGCCC19480.12838552565239025No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGGAGTTCATTTGCAGATACAGGG19440.1281219003430424No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT19430.12805599401570547No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG19120.12601289786825984No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC19020.12535383459489027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18980.12509020928554246No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAAGTTTCTGCTGATACCAGGCTAAGGACCTGCTGCTGACACTCTGA18980.12509020928554246No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18570.12238804986472726No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18330.12080629800864033No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18230.12014723473527077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18190.11988360942592294No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18130.1194881714619012No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18120.11942226513456423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18000.11863138920652078No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAACAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA17980.11849957655184686No Hit
GGACTGGAGTGGGTCTCAGGTATTAGTGGTGGTGGGGATACAACATACTA17820.11744507531445557No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17670.11645648040440122No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17650.1163246677497273No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17570.11579741713103166No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17400.11467700956630342No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17340.11428157160228167No Hit
GGAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17050.11237028810950996No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17050.11237028810950996No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16320.10755912621391217No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTGACCGGAGGTGAAGTACCATACTG16230.10696596926787956No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16130.10630690599451001No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16110.1061750933398361No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCC16040.1057137490484774No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16040.1057137490484774No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15990.10538421741179262No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15330.10103439980755353No Hit
CAGGAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15280.10070486817086875No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGT15260.10057305551619483No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCACTGTCCCTTGG15200.10017761755217311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA200.007024185352.36384247
TTAGATC200.007875035550.871234
ATATCGT551.5322257E-743.6787637
CTTATAC5600.041.201841
TCGCTAA5250.040.90999231
AATATCG603.0611045E-739.9935236
CGCTAAT5300.039.90989332
TCGTATG5550.039.7673840
ATTACGT350.001293163339.76273345
AATGGTA2300.039.4263170
CGTATGC5700.039.3971741
TTATACA5950.039.329022
ACTCGCT5550.039.24530429
ACACTCG5700.038.75705327
GTATCAA29250.038.6290551
CGAGACA5800.038.60978323
GCCGTCT5800.038.46182346
TCTCCGA5850.038.2646511
CACTCGC5800.038.11148528
ATCTCGT5050.037.3771637
Produced by FastQC (version 0.11.5)