FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517305
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52360.34508552993630154No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43140.2843198961316281No Hit
GTATGGTACTTCACCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT42340.2790473899446716No Hit
GTGATATAGGGCCGTGTCCTCGGCTCTCAGGGAGTTCATTTGCAGATACA41770.27529072928646514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41150.2712045369915739No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT29380.1936327897159767No Hit
GTACCATACTGCTGACAGGAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC29270.19290782011527016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28900.19046928600380278No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28550.18816256454700933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26850.17695848889972682No Hit
CTATATCACTGTGCGAGATCTCTTGTTGTGGCCATACGTCCTACTACCGA26550.17498129907961812No Hit
GCCCTATATCACTGTGCGAGATCTCTTGTTGTGGCCATACGTCCTACTAC26470.1744540484609225No Hit
GTATTCCTGTCAGCAGTATGGTACTTCACCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAA24800.16344769179565086No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24770.16324997281363995No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24360.16054781339282478No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23140.15250724145771613No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGAATTCACCTTTAACAACTATGCC23040.1518481781843466No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22800.15026642632825965No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22660.14934373774554227No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22640.14921192509086834No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22470.1480915175261401No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22420.14776198588945533No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22330.1471688289434227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22030.14519163912331404No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAGGTCCTTAGCCTGGTATC21930.14453257584994447No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21800.14367579359456406No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21610.14242357337516187No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21500.14169860377445537No Hit
GTGTATTCCTGTCAGCAGTATGGTACTTCACCTCCGGTCACCTTCGGCCA21100.13906235068097714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20830.13728287984287932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19840.13075815343652067No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19750.13016499649048807No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19500.1285173383070642No Hit
GTAGTAGGACGTATGGCCACAACAAGAGATCTCGCACAGTGATATAGGGC19360.12759464972434678No Hit
TCCTACTACCGATGCTTTTGCTGTCTGGGGCCAAGGGACAGTGGTCACCGTCTCTTCAGCATCCCCGACCAGCCC19170.12634242950494462No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18900.12456295866684682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18830.12410161437548813No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18710.12331073844744464No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAACAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA18600.12258576884673814No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18350.12093811066331422No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18250.12027904738994466No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18220.1200813284079338No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18070.11909273349787948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17960.11836776389717295No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17870.11777460695114035No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17690.11658829305907514No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG17690.11658829305907514No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17660.11639057407706428No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGGAGTTCATTTGCAGATACAGGG17540.1155996981490208No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17430.11487472854831429No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17390.11461110323896645No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17210.11342478934690124No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17120.11283163240086864No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAAGTTTCTGCTGATACCAGGCTAAGGACCTGCTGCTGACACTCTGA17070.11250210076418386No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16810.11078853625342301No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16760.11045900461673823No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16680.10993175399804259No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16530.10894315908798824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16450.1084159084692926No Hit
GGACTGGAGTGGGTCTCAGGTATTAGTGGTGGTGGGGATACAACATACTA16360.10782275152325999No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTGACCGGAGGTGAAGTACCATACTG16310.1074932198865752No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16260.10716368824989043No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15900.10479106046576002No Hit
GGAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15880.10465924781108611No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15400.10149574409891221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG200.00789724850.8349959
GTATCAA31000.036.6580961
TATAAGA1155.820766E-1132.4197242
TCTGGAG18500.031.3738570
ATCAACG36600.030.9269393
TCAACGC37100.030.5101344
TATCAAC37500.030.3684392
AACGCAG37450.030.3154856
TCGGACT450.00489917430.30520631
CAACGCA37500.030.2750635
GGTACGG450.00504462730.1244433
GGTACTA500.00628373628.8004560
ACGCAGA39500.028.742157
ACTCGTA2000.028.70098159
AGAGTAC39900.028.36907211
CGCAGAG40250.028.2065838
CTCGTAT2050.028.09799860
GCAGAGT40950.027.7244199
ATAAGAG1405.7661964E-1026.6278553
AGAGTCG659.6086884E-426.069235
Produced by FastQC (version 0.11.5)