FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407411
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCAAAGCCTGGGT13600.3338152381747179No Hit
GGACAACACCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA9350.22949797624511856No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9150.2245889286249021No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7930.19464373814158184No Hit
GAAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATACA7870.1931710238555169No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7790.19120740480743034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7610.18678926194923554No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC7310.17942569051891089No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7150.17549845242273773No Hit
CTCCTGTGCAGTCTCTGGATTCATCTTCAGTAGTTATGAAATGAACTGGCTCCGCCAGGCTCCAGGGAAGGGG6930.17009850004049965No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6910.169607595278478No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6790.16666216670634815No Hit
TCCACATACTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCACTGTATCTG6650.16322583337219662No Hit
GTCTGGAGGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTCATCTTCAGTAGTTATGAAATGAACTGGCTCCG6540.1605258571810776No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTGGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT6460.158562238132991No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6350.15586226194187197No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC6250.15340773813176375No Hit
GTATGTGGAAGTACCACTCCTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAGCCAGTT6130.15046230955963388No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6000.1472714286064932No Hit
GTCTAGGCCCAGCCACTGTTCCTCACTTCTCGCACAGAAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCA5880.14432600003436333No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5820.14285328574829842No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT5560.13647152384201705No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC5550.1362260714610062No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC5510.13524426193696293No Hit
CCTCTGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA5480.13450790479393046No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC5330.13082611907876812No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA5220.1281261428876491No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5210.12788069050663828No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5120.12567161907754087No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5070.12444435717248675No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5050.1239534524104651No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5010.12297164288642183No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4980.12223528574338935No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGTGTTGTCCCACGCCTGACAGTAATAGTCAGCCT4940.12125347621934607No Hit
GGGTTCCCTGGCCCCAGTAGTCTAGGCCCAGCCACTGTTCCTCACTTCTCGCACAGAAATAAACAGCCGTGTCCT4940.12125347621934607No Hit
CTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAGTGAA4920.12076257145732443No Hit
GTACCAAGGCTCCCCCAGACTCCACCAGCTGCACCTCACACTGGACACCT4890.12002621431429195No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4870.11953530955227032No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG4810.11806259526620538No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4750.11658988098014043No Hit
CTATTACTGTCAGGCGTGGGACAACACCGTGCTATTCGGCGGAGGGACCA4690.11511716669407551No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4680.1148717143130647No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT4680.1148717143130647No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4670.11462626193205387No Hit
GTGCTGGGTTTTCCTGGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC4600.11290809526497811No Hit
GGCCTAGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG4590.1126626428839673No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG4590.1126626428839673No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG4570.11217173812194565No Hit
GTAATAGTCAGCCTCATCCAAAGCCTGGGTCCCCCTGATGGTCAGAGTGG4530.11118992859790236No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT4500.11045357145486989No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA4460.10947176193082661No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCACCTGCTCTGG4430.10873540478779414No Hit
GTTTATTTCTGTGCGAGAAGTGAGGAACAGTGGCTGGGCCTAGACTACTGGGGCCAGGGAACCCTGGTCACCGT4390.10775359526375085No Hit
GACTATTACTGTCAGGCGTGGGACAACACCGTGCTATTCGGCGGAGGGAC4380.10750814288274003No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC4370.10726269050172921No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT4350.10677178573970755No Hit
GCCTTGGTACAGTCTGGAGGGTCCCTGAGACTCTCCTGTGCAGTCTCTGG4350.10677178573970755No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4330.10628088097768593No Hit
CAGTAATAGTCAGCCTCATCCAAAGCCTGGGTCCCCCTGATGGTCAGAGT4280.10505361907263182No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGGCCCAGCCA4260.10456271431061018No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4150.10186273811949113No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4130.10137183335746948No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4080.10014457145241537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)