FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407411
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCAAAGCCTGGGT13160.32301533341024175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11970.2938065000699539No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9730.2388251667235298No Hit
GGACAACACCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA9660.23710700005645405No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7330.17991659528093254No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7270.1784438809948676No Hit
CTCCTGTGCAGTCTCTGGATTCATCTTCAGTAGTTATGAAATGAACTGGCTCCGCCAGGCTCCAGGGAAGGGG7020.17230757146959705No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6910.169607595278478No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC6880.16887123813544555No Hit
GAAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATACA6290.15438954765580704No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTGGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT6270.1538986428937854No Hit
GTCTGGAGGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTCATCTTCAGTAGTTATGAAATGAACTGGCTCCG6020.14776233336851485No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6020.14776233336851485No Hit
TCCACATACTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCACTGTATCTG5900.14481690479638498No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5890.14457145241537414No Hit
GTCTAGGCCCAGCCACTGTTCCTCACTTCTCGCACAGAAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCA5880.14432600003436333No Hit
GTATGTGGAAGTACCACTCCTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAGCCAGTT5870.14408054765335251No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5770.1416260238432443No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5650.13868059527111443No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5610.13769878574707115No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA5400.13254428574584387No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5300.13008976193573565No Hit
CCTCTGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA5180.12714433336360578No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5010.12297164288642183No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA4970.12198983336237854No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4960.12174438098136772No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC4960.12174438098136772No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC4930.12100802383833524No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC4890.12002621431429195No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4860.11928985717125949No Hit
GTGCTGGGTTTTCCTGGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC4830.11855350002822702No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGTGTTGTCCCACGCCTGACAGTAATAGTCAGCCT4760.11683533336115128No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCACCTGCTCTGG4750.11658988098014043No Hit
GGCCTAGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG4730.11609897621811881No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG4700.11536261907508633No Hit
CTATTACTGTCAGGCGTGGGACAACACCGTGCTATTCGGCGGAGGGACCA4680.1148717143130647No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC4660.11438080955104306No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4640.1138899047890214No Hit
GGGTTCCCTGGCCCCAGTAGTCTAGGCCCAGCCACTGTTCCTCACTTCTCGCACAGAAATAAACAGCCGTGTCCT4630.11364445240801058No Hit
GTTTATTTCTGTGCGAGAAGTGAGGAACAGTGGCTGGGCCTAGACTACTGGGGCCAGGGAACCCTGGTCACCGT4600.11290809526497811No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4590.1126626428839673No Hit
GCCTTGGTACAGTCTGGAGGGTCCCTGAGACTCTCCTGTGCAGTCTCTGG4580.11241719050295647No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4570.11217173812194565No Hit
CTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAGTGAA4570.11217173812194565No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGGCCCAGCCA4530.11118992859790236No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG4480.10996266669284825No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC4470.10971721431183744No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC4430.10873540478779414No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG4410.1082445000257725No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4330.10628088097768593No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG4320.1060354285966751No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4290.10529907145364263No Hit
GACTATTACTGTCAGGCGTGGGACAACACCGTGCTATTCGGCGGAGGGAC4270.10480816669162099No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT4170.10235364288151277No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4110.10088092859544785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTA150.002449770768.31283613
GTATCAA7950.042.560531
TTTTTCG350.001413855639.04069515
CCTAGAG350.001417272739.021533
GTGGTAT1150.038.6353151
TCTGTAC451.0634609E-437.93763
ACGGGTA754.393405E-836.4200945
TATCAAC9350.036.1522982
GACAGGT1051.8189894E-1135.8048931
AACTACG400.002469171534.84955648
ATCAACG9800.034.4922453
TTTCGTC400.002721593234.16480317
CAACGCA9900.034.1438375
AACGCAG9950.033.9722636
TCAACGC10000.033.8024024
ACGGGAC553.457457E-431.0398543
GCAGAGT11000.031.0398549
CTGCGAT450.004833530630.38363625
CAGAGTA11250.030.35007910
CGCAGAG11200.030.1807148
Produced by FastQC (version 0.11.5)