FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475576
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23650.49729170521641125No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17460.36713374939021315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15830.3328595219270947No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15770.33159789392231737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13850.2912257977694417No Hit
GTATTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC13370.28113277373122275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13140.27629653304624285No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12920.2716705636953925No Hit
GCGTAATACGTTCGGCCCGGGGACCAAGGTGGAAATCAAACGAACTGTGG12110.25463858563089814No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11640.24475583292680875No Hit
ATAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCATCTTCAGGGAGA11450.2407606775783471No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT11310.23781687890053324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11220.23592443689336723No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCT11210.23571416555923766No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG11130.23403199488620116No Hit
GTCTCTGGTGGCTCCATCAGAAGTAGTAGTTACCTCTGGGGCTGGATCCG11090.2331909095496829No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10270.2159486601510589No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10010.21048160546369032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9940.2090097061247834No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9640.2027015661008966No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9500.19975776742308274No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAATACG9380.19723451141352802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9180.1930290847309368No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT9150.19239827072854812No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9070.19071610005551165No Hit
GTTTATTACTGTCAGCAGCGTAATACGTTCGGCCCGGGGACCAAGGTGGA8820.18545931670227261No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8690.18272578935858833No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA8620.1812538900196814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8620.1812538900196814No Hit
GTATATTATTGTGCGAGACATCTTTACTATGATAGTAGTGGTTATTCTCCCCTTGACTACTGGGGCCAGGGAACC8540.1795717193466449No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8430.17725873467121975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8430.17725873467121975No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8140.17116086598146246No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8130.17095059464733292No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8080.1698992379766851No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7790.16380136928692784No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7450.1566521439265228No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCGGGCCGAACGTATTACGCTGCTGACAGTAATAAACTG7420.15602132992413412No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCTCCTGATATATGATG7340.15433915925109762No Hit
CCTGATATATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT7020.14761047655895168No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGAAGTAGTAGTTACCTCTGGG6930.1457180345517856No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6850.14403586387874914No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC6760.14214342187158308No Hit
GCATTATAGTGGGAGCACCTACCACAACCCGTCCCTCAAGAGTCGGGTCACCATATCCGTAGACACGTCCAAGAA6690.14067152253267615No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6530.13730718118660318No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC6470.13604555318182582No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6460.13583528184769625No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6430.13520446784530757No Hit
CAATAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCATCTTCAGGGA6270.1318401264992346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6260.13162985516510506No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6200.13036822716032767No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6130.12889632782142077No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCTCCTGATATATGATGCATC6110.12847578515316163No Hit
GTGTTAGCAGCTACTTAGCCTGGTATCAACAGAAACGTGGCCAGGCTCCC6080.12784497115077295No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6060.12742442848251384No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC6000.12616280047773648No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5870.12342927313405219No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG5820.12237791646340437No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5820.12237791646340437No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5800.12195737379514526No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5760.12111628845862701No Hit
GATATATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA5690.11964438911972008No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5500.11564923377125844No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5430.11417733443235151No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5360.11270543509344458No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5290.11123353575453766No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5290.11123353575453766No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5260.11060272175214897No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5250.11039245041801941No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5240.11018217908388984No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5230.10997190774976029No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA5220.10976163641563073No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT5120.10765892307433512No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5120.10765892307433512No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5100.10723838040607599No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC5070.1066075664036873No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC5000.10513566706478038No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4960.10429458172826216No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA4950.10408431039413259No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT4930.10366376772587348No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4850.10198159705283698No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGGGGAGAATAACCACTACTATCATAGTAAAGATGTCTCGCACAATAATATA4820.1013507830504483No Hit
ATATTATTGTGCGAGACATCTTTACTATGATAGTAGTGGTTATTCTCCCCTTGACTACTGGGGCCAGGGAACC4820.1013507830504483No Hit
TTATTACTGTCAGCAGCGTAATACGTTCGGCCCGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT4810.10114051171631873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACC150.002460312368.240673
CTCGTGT200.006869864752.65308454
ACGAAAC200.007545746451.41837729
ATAGCCG200.00762484351.2829522
TACTGAC200.007682173551.1858867
TAAGTCA805.8385143E-541.9925670
TACGGAT4400.039.70205770
ATGTGAT700.001533725238.393270
ATCAACG12900.036.500823
TCAACGC13050.036.0812724
CAACGCA13350.035.270465
AACGCAG13600.034.6257486
TCCGCAC400.002718366434.17423220
AGAGTAC14200.032.92237511
CGCAGAG14400.032.702098
ACGCAGA14450.032.588947
GCAGAGT14750.032.3888059
TATTACA751.813889E-631.8523452
TCGCTGC1400.031.689832
GACAGCT652.4829302E-531.5288431
Produced by FastQC (version 0.11.5)