FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045939
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGAGTCCTCGGCTCTCAGGCTGTTCACTTCCAGATACA37830.36168457242726393No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37160.355278845133416No Hit
GTATAATGACTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT32040.306327615664011No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27950.26722399681052145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27730.265120623669258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27080.25890611211552494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22990.21980249326203535No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT21760.20804272524497128No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20240.19351032899624165No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20080.19198060307532275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19960.19083330863463355No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19830.18959040632388696No Hit
GTATATTACTGTGCGAAGGGGCAGCTATACTTATCGGGTTTTCCTTTTGA19330.18481001282101536No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19150.1830890711599816No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19140.18299346328992416No Hit
CTCCTGCAGGGCCAGTCAGAGCGTTAGTAGCAGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18700.17878671700739718No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17550.16779181195079254No Hit
CACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG17300.16540161519935676No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTTAGTAG17160.1640631050185527No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTTAGTAGCAGCTTAGCCTGGTA17150.1639674971484953No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16880.16138608465694462No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAATTAGTG16150.15440671014275212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16100.15392867079246497No Hit
CTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16090.15383306292240753No Hit
CATCTATGGTGCATACACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15960.1525901606116609No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15720.15029557173028255No Hit
GTGCATACACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGG15670.1498175323799954No Hit
ATCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA15410.14733172775850217No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGATACCAGTGGCCCTGGT15390.14714051201838732No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15030.14369862869631977No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14760.1411172162047691No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14750.14102160833471167No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14530.13891823519344818No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14480.13844019584316103No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14370.13738850927252927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14240.13614560696178268No Hit
CTCCTGTGCAGGCTCTGGATTGACCTTTAGCAACTATGCCATGAACTGGGTCCGCCAGACTCCAGCGAAGGGG14070.13452027317080634No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14050.13432905743069146No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14010.13394662595046172No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13940.13327737086005972No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13910.1329905472498874No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13570.12973987966793474No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13330.1274452907865564No Hit
GTATGTGATACCACCACTACCACTAATAGTCGAGACCCACTCCAGCCCCTTCGCTGGAGTCTGGCGGACCCAGTT13280.12696725143626922No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC13150.1257243491255226No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGTGGACGTTCGGCCAAGGGAC13020.124481446814776No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12980.12409901533454629No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12640.12084834775259361No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12590.12037030840230645No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC12570.12017909266219158No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA12250.11711964082035375No Hit
GTATCTGGAAGTGAACAGCCTGAGAGCCGAGGACTCGGCCGTATATTACT12180.11645038572995176No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12140.11606795424972202No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12090.11558991489943488No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11700.11186120796719502No Hit
GTGTATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGCTGCTACTAACG11680.11166999222708017No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11550.11042708991633354No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11450.10947101121575922No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11360.10861054038524234No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11230.10736763807449574No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11150.10660277511403628No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAATTAGTGCTGACGCAGTC11040.10555108854340454No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10920.10440379410271536No Hit
GGATACCAGTGGCCCTGGTGTATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG10850.10373453901231333No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10820.10344771540214104No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10770.10296967605185389No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGTTGGGATACCA10570.10105751865070525No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10540.10077069504053296No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10470.10010143995013093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT100.00866594135.5831370
CGTTTAA354.7931502E-577.4760870
CGGGTAT209.198113E-567.9310517
GATCGAT250.007273487751.9019469
ATTGGTG554.48166E-449.3029670
CCGTTTA408.5475005E-443.25161469
AATGGTA2800.038.7380470
GTATCAA19750.038.69491
AAAACCG750.00206197936.15550270
GACAGGT2050.034.7939531
TATCAAC22350.034.1656882
TACACTC400.002813970733.937925
TCTGGAG13600.033.3973270
ATCAACG23000.032.901883
CAACGCA22950.032.8288355
TCAACGC23000.032.7543374
AACGCAG23600.031.9277066
ACCTAAT850.003808478831.90191770
ACGGGTA1209.276846E-1131.13208416
AGAGTAC25300.030.05067611
Produced by FastQC (version 0.11.5)