FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903918
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27020.29892091981794805No Hit
GAAATATACGGCCGTGTCCTCGGCGCTCAGGCGGCTCATTTGCAGATGCA25220.2790076090972854No Hit
GGACTGAACAGAGAGAACTCACCATGGGGTTTGGGCTGAGCTGGCTTTTT23880.26418325556079203No Hit
GCCTTGGGCTGACTGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC22820.25245652813640174No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21600.23895972864795256No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20670.22867118477561016No Hit
CTCCTGCACCGGGAGCACCTCCAACATCGGGGCGAGTTATGATGTACACTGGTATCAGCAACTTCCAGGAAAAGC19800.21904641792728988No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT19120.21152361165503952No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTAACAGTTATGCC19080.21108109363902477No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19050.21074920512701373No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17750.1963673696065351No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17260.19094652391035472No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17110.18928708135029948No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16840.18630008474220006No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16820.1860788257341927No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCTTGGTCCCTC16310.18043672103000494No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16280.1801048325179939No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15460.17103321318969197No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15440.17081195418168463No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15440.17081195418168463No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15380.17014817715766253No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15230.1684887345976073No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14990.16583362650151895No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14710.16273600038941585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14600.16151907584537536No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14530.16074466931734957No Hit
GTATATTTCTGTGCGAAGCAGAATTACTTTAATAGCAATGATTTTGGGCACTTTGACTACTGGGGCCAGGGAAAC14530.16074466931734957No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14310.15831082022926857No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14180.1568726366772207No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAACAGTTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG14160.15665137766921336No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13460.14890731238895563No Hit
GTCTTAGGGACCCCCCAGGCTGTACCAAGTCTCCCCCTGATTCCAACAAT13330.14746912883690777No Hit
GGCCATCACTGACCTCCAGGCTGAAGATGATGGTCATTATTACTGCCATT13320.1473584993329041No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13070.14459276173281205No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12680.14027821107666846No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12400.13718058496456537No Hit
GTGTGAGGTGAAATTGTTGGAATCAGGGGGAGACTTGGTACAGCCTGGGGGGTCCCTAAGACTCTCCTGTGCAGC12340.13651680794054327No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12270.1357424014125175No Hit
GTGATGGCCAGGGAGGCTGTGGTGTCAGACTTGGAGCCAGAGAATCGGTC12180.13474673587648436No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12130.13419358835646597No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12070.13352981133244388No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12000.13275540480441808No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11990.1326447753004144No Hit
GTCATAGGAATGGCAGTAATAATGACCATCATCTTCAGCCTGGAGGTCAG11980.13253414579641074No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11810.13065344422834813No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11690.12932589018030397No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT11690.12932589018030397No Hit
CCTCCAACATCGGGGCGAGTTATGATGTACACTGGTATCAGCAACTTCCA11610.1284408541482745No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11540.12766644762024876No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGTTTCCCTGGCCCCAGTAGTCAAAGTGCCCAAAAT11380.12589637555618982No Hit
GGGCAGAGGGTCACCATCTCCTGCACCGGGAGCACCTCCAACATCGGGGCGAGTTATGATGTACACTGGTATCAG11330.12534322803617143No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11140.12324126746010146No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11080.12257749043607938No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11010.12180308390805361No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGT10900.12058615936401311No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10870.12025427085200206No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGGGTTTGGGCTGAGCTGGC10800.11947986432397628No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCATAACTGT10740.1188160872999542No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGTT10730.11870545779595053No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10680.11815231027593211No Hit
GCCTGGGGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTAACAGTTATGCCATGAGTTGGGTCCG10670.11804168077192843No Hit
GAACAGAGAGAACTCACCATGGGGTTTGGGCTGAGCTGGCTTTTTCTTGT10580.11704601523589528No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10540.11660349721988056No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10540.11660349721988056No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT10210.11295272358775907No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC10110.11184642854772225No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10080.1115145400357112No Hit
GGGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAGAGGTGCCCA10060.11129328102770385No Hit
GACTGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGCACCCAGG10010.11074013350768544No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10010.11074013350768544No Hit
GGGCTGGAGTGGGTCTCAACTATTAGTGGTGATGGTTATAGTTCATACTA9890.10941257945964125No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9840.10885943193962284No Hit
GTCTGACACCACAGCCTCCCTGGCCATCACTGACCTCCAGGCTGAAGATG9770.10808502541159706No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9750.10786376640358972No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9690.10719998937956761No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGTTTCCCTGG9680.10708935987556394No Hit
TGGTTATAGTTCATACTACGCAGACTCCGTTAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAGCACC9650.10675747136355289No Hit
TCCTATGACAGCAGCCTGGGTGCTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTCAGTCAGCCCAAGG9550.10565117632351606No Hit
GTATGAACTATAACCATCACCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT9550.10565117632351606No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9540.10554054681951239No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT9530.10542991731550871No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9470.10476614029148662No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9450.10454488128347926No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9380.10377047475545348No Hit
GATGTACACTGGTATCAGCAACTTCCAGGAAAAGCCCCCAAAGTCGTCAT9350.10343858624344243No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9300.10288543872342401No Hit
CCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC9180.10155788467537984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3750.075.9851670
ATCTCGT2900.049.67141737
TCTCGTA3000.049.2581238
CGTATGC3250.048.9182141
TCGTATG3150.048.1925640
TACGCTG4050.047.9053329
ATGCCGT3350.047.68373544
TTATACA4200.047.6460082
GTATGCC3650.047.4224242
CTACGCT4100.047.2843728
CCGTCTT3250.047.2790247
TATACAC4250.047.0854643
ATACACA4450.046.493654
CGAGACT4400.046.2690523
CCCACGA4350.046.0055919
TACACAT4450.045.7314575
CGTCTTC3150.045.5474248
ACGAGAC4400.045.49034522
CACGAGA4400.045.48531321
ACGCTGC4350.045.4142530
Produced by FastQC (version 0.11.5)