FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903918
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40920.4526959303830657No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28210.31208583079438623No Hit
GAAATATACGGCCGTGTCCTCGGCGCTCAGGCGGCTCATTTGCAGATGCA22490.24880575450428027No Hit
GGACTGAACAGAGAGAACTCACCATGGGGTTTGGGCTGAGCTGGCTTTTT21970.24305302029608877No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19890.220042083463323No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19680.2177188638792457No Hit
CTCCTGCACCGGGAGCACCTCCAACATCGGGGCGAGTTATGATGTACACTGGTATCAGCAACTTCCAGGAAAAGC19370.21428934925513154No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG19110.21141298215103582No Hit
GCCTTGGGCTGACTGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC18770.20765157901491063No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTAACAGTTATGCC17940.19846933018260507No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17030.18840204531827004No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16660.18430875367013377No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16230.17955168499797547No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16110.1782241309499313No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15460.17103321318969197No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15460.17103321318969197No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15320.16948440013364044No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15130.16738243955757048No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14950.1653911084855042No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13880.15355375155711026No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13870.1534431220531066No Hit
GTATATTTCTGTGCGAAGCAGAATTACTTTAATAGCAATGATTTTGGGCACTTTGACTACTGGGGCCAGGGAAAC13790.15255808602107712No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAACAGTTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG13680.15134116147703663No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13670.15123053197303296No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13640.15089864346102191No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13140.14536716826083781No Hit
GTCTTAGGGACCCCCCAGGCTGTACCAAGTCTCCCCCTGATTCCAACAAT13130.14525653875683414No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT12810.14171639462871632No Hit
GGCCATCACTGACCTCCAGGCTGAAGATGATGGTCATTATTACTGCCATT12430.1375124734765764No Hit
GTGTGAGGTGAAATTGTTGGAATCAGGGGGAGACTTGGTACAGCCTGGGGGGTCCCTAAGACTCTCCTGTGCAGC12380.13695932595655802No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12350.13662743744454697No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12290.13596366042052488No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12270.1357424014125175No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12220.13518925389249908No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12120.1340829588524623No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11790.13043218522034078No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11770.1302109262123334No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC11670.1291046311722966No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11610.1284408541482745No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCTTGGTCCCTC11440.12656015258021192No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11380.12589637555618982No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11260.12456882150814566No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11160.12346252646810883No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11090.12268811994008307No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGTTTCCCTGGCCCCAGTAGTCAAAGTGCCCAAAAT10880.12036490035600574No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10840.11992238233999103No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCATAACTGT10810.11959049382797998No Hit
GAACAGAGAGAACTCACCATGGGGTTTGGGCTGAGCTGGCTTTTTCTTGT10780.11925860531596894No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGTT10720.11859482829194683No Hit
GGGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAGAGGTGCCCA10720.11859482829194683No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10650.11782042176392106No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10620.11748853325191003No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10540.11660349721988056No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC10500.11616097920386585No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10450.11560783168384743No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10450.11560783168384743No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10400.11505468416382902No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10370.11472279565181798No Hit
GGGCAGAGGGTCACCATCTCCTGCACCGGGAGCACCTCCAACATCGGGGCGAGTTATGATGTACACTGGTATCAG10320.11416964813179957No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10020.11085076301168911No Hit
GCCTGGGGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTAACAGTTATGCCATGAGTTGGGTCCG9980.11040824499567439No Hit
GTCATAGGAATGGCAGTAATAATGACCATCATCTTCAGCCTGGAGGTCAG9920.1097444679716523No Hit
CCTCCAACATCGGGGCGAGTTATGATGTACACTGGTATCAGCAACTTCCA9860.10908069094763022No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9620.10642558285154184No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9580.10598306483552712No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9460.10465551078748293No Hit
TCCTATGACAGCAGCCTGGGTGCTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTCAGTCAGCCCAAGG9420.10421299277146821No Hit
TGGTTATAGTTCATACTACGCAGACTCCGTTAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAGCACC9420.10421299277146821No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGGGTTTGGGCTGAGCTGGC9410.10410236326746451No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9400.10399173376346084No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGTTTCCCTGG9400.10399173376346084No Hit
GTCTGACACCACAGCCTCCCTGGCCATCACTGACCTCCAGGCTGAAGATG9400.10399173376346084No Hit
GATGTACACTGGTATCAGCAACTTCCAGGAAAAGCCCCCAAAGTCGTCAT9370.1036598452514498No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9350.10343858624344243No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT9300.10288543872342401No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9230.10211103219539826No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9230.10211103219539826No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC9180.10155788467537984No Hit
GTATGAACTATAACCATCACCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT9100.10067284864335038No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC9060.10023033062733566No Hit
GGGCTGGAGTGGGTCTCAACTATTAGTGGTGATGGTTATAGTTCATACTA9040.1000090716193283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAA252.5302253E-455.36087842
TCTATGT252.754448E-454.41745429
TAACGCA200.007062220552.2906146
GTACGTA250.00719604352.0418969
CGGGTAT200.007883673550.85545317
GTGGTAT2650.042.228971
GTATCAA18450.038.0466461
GGGTTAT400.002824676733.911141
TATACAA502.0658961E-433.8923845
TATCAAC21450.032.7091452
CTAGTTC553.428235E-431.08985935
ATCAACG23000.030.6505013
CAACGCA23050.030.4369775
AACGCAG23000.030.3574646
TCAACGC23200.030.2401874
CTCGCTA902.3090797E-730.15825823
GCTTGGG902.3191751E-730.1432381
GACAGGT1401.8189894E-1129.0666921
AATGGTA1903.6026177E-728.65826470
CGCAGAG24250.028.6528788
Produced by FastQC (version 0.11.5)