FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568008
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44020.28073836357977766No Hit
GTCTACGACCTGAGGACACGGCCTTATATTACTGTGCGAAAGACATAGCT41480.2645394666353743No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39560.2522946311498411No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31700.2021673358809394No Hit
GATCTATGCTGCTTCCCGTTTGCAAAGTGGGGTCCCGTCAAAGTTCAGCGGCAGTGGTTATGGCACACTTTTCA30550.19483318962658355No Hit
GCCTTATATTACTGTGCGAAAGACATAGCTGGTGCAGGATATTCCTACGC29490.18807302003561205No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29030.18513936153386973No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27880.17780521527951387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27020.17232054938495212No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26960.1719378982760292No Hit
GTAATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTTCAGCCACA26650.16996086754659415No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26520.16913179014392785No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26520.16913179014392785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24520.1563767531798307No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT24300.15497369911378003No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24270.1547823735593186No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23840.1520400406120377No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23130.14751200248978322No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22960.14642782434783494No Hit
GGATATTCCTACGCGGGCATTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC22900.14604517323891206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22690.14470589435768183No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22450.1431752899219902No Hit
GCATAATAAGTACCCCCTGACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT21570.13756307365778744No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21440.13673399625512114No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21380.13635134514619823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21370.13628756996137775No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21360.13622379477655727No Hit
CTTTTCACTCTCACCATCAGGAACCTGCAGCCTGAAGACTTTGCAACCTACTTCTGTCAACAGCATAATAAGTAC20620.13150443109984133No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20610.13144065591502085No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20440.13035647777307258No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20220.1289534237070219No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20180.12869832296773995No Hit
CAGTAATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTTCAGCCA19590.1249355870633313No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19460.124106509660665No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC19310.12314988188835772No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19290.12302233151871675No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18130.11562441007954043No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18100.11543308452507896No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17900.11415758082866925No Hit
ATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTTCAGCCACAGTA17760.11326472824118244No Hit
ATATTACTGTGCGAAAGACATAGCTGGTGCAGGATATTCCTACGCGGGCATTGACTCTTGGGGCCAGGGAACC17730.11307340268672099No Hit
GGAATATCCTGCACCAGCTATGTCTTTCGCACAGTAATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTT17630.11243565083851612No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC17630.11243565083851612No Hit
GTCTTCAGGCTGCAGGTTCCTGATGGTGAGAGTGAAAAGTGTGCCATAACCACTGCCGCTGAACTTTGACGGGAC17290.11026729455461962No Hit
GTCTGGGGGAGGCCTGGTAGAACCTGGCGGGTCCCTGAGACTTTCCTGTG17240.1099484186305172No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17120.10918311641267137No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16870.10758873679215923No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16830.1073336360528773No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16630.10605813235646756No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16370.10439997755113495No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16340.10420865199667348No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT16290.10388977607257105No Hit
GTGGGAGACAGCGTCACCATCACTTGTCGGGCGACTCAGGGCATTAAGGATCATTTAGCCTGGTTTCAGCAGAAA16270.10376222570293009No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16210.10337957459400718No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16080.10255049719134086No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16040.10229539645205891No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16020.10216784608241794No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15970.10184897015831551No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG15950.10172141978867454No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15840.10101989275564921No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15700.1001270401681624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT309.246047E-657.374939
TACGATC200.007618881351.29862234
TCGTATG4600.050.98808740
CGTATGC4550.050.8695341
TACCGAT200.00787684950.868357
CTTATAC4800.049.4711151
CGCTAAT4500.049.27031732
TATCGTA352.3248458E-549.0990138
TCGCTAA4750.048.0671131
GCCGTCT5050.046.2264146
ACTCGCT5050.045.79837429
ATGCCGT5150.045.17191344
ACACTCG5150.044.85886427
TATACAC5450.044.795773
ATCTCCG5150.044.77731710
GCTAATC4500.044.77399433
CACGAGA5250.044.58031521
TTATACA5250.044.56612
TGGTATC4650.044.482762
CGAGACA5200.044.36940823
Produced by FastQC (version 0.11.5)