FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568008
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45830.29228167203228556No Hit
GTCTACGACCTGAGGACACGGCCTTATATTACTGTGCGAAAGACATAGCT43080.274743496206652No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40630.25911857592563303No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39330.2508278018989699No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30220.1927286085275075No Hit
GATCTATGCTGCTTCCCGTTTGCAAAGTGGGGTCCCGTCAAAGTTCAGCGGCAGTGGTTATGGCACACTTTTCA29070.18539446227315168No Hit
GCCTTATATTACTGTGCGAAAGACATAGCTGGTGCAGGATATTCCTACGC28730.18322610598925515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27090.1727669756786955No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25920.1653052790546987No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25880.16505017831541677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25790.1644762016520324No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25730.16409355054310948No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24220.15446349763521613No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23650.15082831210044848No Hit
GTAATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTTCAGCCACA23000.14668292508711692No Hit
GGATATTCCTACGCGGGCATTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC21870.13947632920240202No Hit
CTTTTCACTCTCACCATCAGGAACCTGCAGCCTGAAGACTTTGCAACCTACTTCTGTCAACAGCATAATAAGTAC21140.13482074071050656No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21020.13405543849266077No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20900.13329013627481492No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20840.13290748516589201No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20700.13201463257840523No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20630.13156820628466181No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20590.13131310554537987No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20460.13048402814271357No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20130.12837944704363755No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19660.1253820133570747No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19010.12123662634374315No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18980.12104530078928169No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18860.12027999857143586No Hit
GCATAATAAGTACCCCCTGACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT18800.11989734746251295No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18720.11938714598394908No Hit
GTCTGGGGGAGGCCTGGTAGAACCTGGCGGGTCCCTGAGACTTTCCTGTG18680.11913204524466711No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18170.11587951081882236No Hit
ATATTACTGTGCGAAAGACATAGCTGGTGCAGGATATTCCTACGCGGGCATTGACTCTTGGGGCCAGGGAACC17790.11345605379564389No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17740.11313717787154148No Hit
CAGTAATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTTCAGCCA17130.10924689159749185No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17120.10918311641267137No Hit
GTCTTCAGGCTGCAGGTTCCTGATGGTGAGAGTGAAAAGTGTGCCATAACCACTGCCGCTGAACTTTGACGGGAC17070.10886424048856894No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC17050.10873669011892796No Hit
GTGGGAGACAGCGTCACCATCACTTGTCGGGCGACTCAGGGCATTAAGGATCATTTAGCCTGGTTTCAGCAGAAA16970.10822648864036406No Hit
ATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTTCAGCCACAGTA16190.1032520242243662No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16100.10267804756098184No Hit
GGAATATCCTGCACCAGCTATGTCTTTCGCACAGTAATATAAGGCCGTGTCCTCAGGTCGTAGACTGTTCATGTT16060.10242294682169988No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16020.10216784608241794No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16020.10216784608241794No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC16000.10204029571277698No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15870.10121121831011066No Hit
CTCCAGAGACAACGCCAAGAATTTACTGTGGCTGAACATGAACAGTCTACGACCTGAGGACACGGCCTTATATTA15850.1010836679404697No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15750.10044591609226483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)