FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771493
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26000.3370088905537704No Hit
ATATAATACTTATTCTCCTGCTTTTGGCCAGGGGACCATGCTGGAGATCAAACGAACTGTGGCTGCACCAT24940.3232692973235013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23670.3068077092079902No Hit
GTATTATATTGTTGGCAAGAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC21840.28308746806516716No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20830.2699959688551938No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGT18340.2377208866444673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17670.22903642677250474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16080.2084270369271011No Hit
ATGTATTACTGTGCGAGAGATCACAGTAGGTGGGAGCTATGGCCGAAAGAAACTGACTACTGGGGCCAGGGAACC15640.2027238095484988No Hit
ATTAATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA14360.18613260262892858No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14070.18237365731121344No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13800.1788739496016166No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13750.1782258555813209No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12930.1675971136484712No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12410.16085693583739583No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12180.15787570334403553No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12110.15696837171562153No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11650.15100590672890096No Hit
GTATTACTGTGCGAGAGATCACAGTAGGTGGGAGCTATGGCCGAAAGAAACTGACTACTGGGGCCAGGGAACC11620.15061705031672357No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11380.14750619901930412No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11380.14750619901930412No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11240.14569153576247615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11160.14465458533000297No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11030.14296954087723415No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10890.14115487762040613No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG10810.140117927187933No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10780.13972907077575558No Hit
ATGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT10700.13869212034328243No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCATGTATTA10630.13778478871486843No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10520.13635898187021786No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA10350.13415546220121247No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10320.13376660578903501No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10290.1333777493768576No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10180.13195194253220702No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTAGCAA9840.12754490319419617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9680.1254710023292499No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9660.12521176472113163No Hit
CAGTAATACATGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA9430.12223053222777136No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9220.11950853734252935No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9180.11899006212629279No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9100.11795311169381965No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9060.11743463647758308No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9030.11704578006540564No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8900.11536073561263681No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAGCTGGTTGGCCTGGTATCAGCAGAAA8900.11536073561263681No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCATGGTCCCCTGGCCAAAAGCA8810.11419416637610451No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8700.11276835953145393No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8500.1101759834502711No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA8480.1099167458421528No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8340.10810208258532482No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8330.10797246378126568No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8320.10784284497720653No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8070.10460237487572797No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7970.10330618683513655No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7830.10149152357830854No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7740.10032495434177627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA350.001446403938.8647041
TATACCC350.001450558138.8420335
GTGGTAT2050.038.1537671
CGGCAAA700.001599049838.0745770
ACCTTTG700.001599049838.0745770
GTATCAA17900.035.9064251
GCTTGTG652.5606068E-531.3907221
TCGGCAA703.5631776E-430.88378769
TATCAAC20750.030.7948382
TATAGGC450.00480377630.42541331
TCAACGC21500.030.0348284
AACGCAG22050.029.9041376
CAACGCA21700.029.7580075
ATCAACG21500.029.7186723
CGCAGAG23450.028.1188168
ACGCAGA23400.028.0336487
TTAACGG500.00791503327.46893735
AGAGTAC24300.026.71561811
CAGAGTA24650.026.47417610
GCAGAGT24950.026.2920789
Produced by FastQC (version 0.11.5)