FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997738
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTGAGACTGGAGTCCACTGAAGGCCAGGGAGG59780.5991552892643159No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGG53470.5359122334721139No Hit
GTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCC46750.4685598824541112No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40670.40762204105687067No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCT38000.3808615087327535No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATG36980.3706383840246638No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32460.325335909828031No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCACTGGCTCCAAG29650.2971722035243721No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGG28970.2903567870523123No Hit
GTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC28820.2888533863599462No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28330.2839422774315502No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC27080.271413938328499No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26170.2622933074614779No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCAC25160.2521704094662126No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23870.23924116351186384No Hit
GATTATTACTTTGGATGAGGAGTTTGGGGCCCGTTCCCGGGAGTTGCTGG23400.23453050800911662No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23270.2332275607423993No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23180.23232552032697962No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22460.22510919700362222No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20450.204963627725916No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19560.19604345028454365No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19480.19524163658194837No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19280.19323710232546018No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18960.1900298475150791No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18930.18972916737660586No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18840.1888271269611862No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG18720.1876244064072933No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATCCTGTAAATTGGTACCAGCAACTCCCGGGAACGGGCCC18600.1864216858534004No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18270.1831142043301949No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17580.17619856114531068No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17560.17599810771966187No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17440.17479538716576895No Hit
CCCCTGAGGGCCGCTGATTATTACTTTGGATGAGGAGTTTGGGGCCCGTTCCCGGGAGTTGCTGGTACCAATTTA17310.17349243989905166No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAAT17280.17319175976057843No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17230.1726906261964564No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16990.17028518508867058No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16880.16918269124760207No Hit
CCTCAGGGGTCCCTGACCGATTCACTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCTTCAGTGGACTCCAGT16870.16908246453477765No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15660.1569550322830242No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15650.1568548055701998No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT15360.15394823089829193No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15260.15294596377004785No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14850.1488366685442471No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14820.14853598840577387No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14700.14733326785188097No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG14540.1457296404466904No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGC14370.14402578632867546No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14030.14061807809264557No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13950.1398162643900503No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13720.1375110499950889No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13580.13610787601554716No Hit
AGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG13460.13490515546165427No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13300.13330152805646373No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12870.12899177940501413No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACAC12770.12798951227677005No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTG12710.1273881519998236No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG12540.12568429788180865No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC12540.12568429788180865No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATCCT12510.12538361774333542No Hit
ACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC12450.12478225746638898No Hit
CCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATC12450.12478225746638898No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC12260.12287794992272522No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12260.12287794992272522No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12250.1227777232099008No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12240.12267749649707638No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12140.1216752293688323No Hit
GTCTGGCACCTCAGCCTCCCTGGCCTTCAGTGGACTCCAGTCTCAGGATG12080.12107386909188585No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC12060.12087341566623704No Hit
GTTTGGGGCCCGTTCCCGGGAGTTGCTGGTACCAATTTACAGGATTATTT12050.12077318895341262No Hit
GAAGTACACAGCCGTGTCCTCGACTTTCAGGCTGTTCATTTGCAGATACA11920.1194702416866953No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11900.11926978826104649No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTGAGACTGGAGTCCAC11850.11876865469692445No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11820.11846797455845122No Hit
CCCTAAGACTCTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGCTTTGGC11750.11776638756868035No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11650.11676412044043627No Hit
GTCCCTGACCGATTCACTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11590.11616276016348981No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTG11540.11566162659936777No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAG11460.1148598128967725No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11450.11475958618394808No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11330.11355686563005518No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA11190.11215369165051346No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTACTTTGGATGAGGAGTTTGGGGCCCGTTCCCGGGAGTTGCTGGTA11020.1104498375324985No Hit
GTGCCAGACTTGGAGCCAGTGAATCGGTCAGGGACCCCTGAGGGCCGCTG10780.1080443964247127No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10550.1057391820297513No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10530.10553872860410247No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT10520.10543850189127807No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10450.1047369149015072No Hit
GCCTCATCCTGAGACTGGAGTCCACTGAAGGCCAGGGAGGCTGAGGTGCC10440.10463668818868281No Hit
GAAATAATCCTGTAAATTGGTACCAGCAACTCCCGGGAACGGGCCCCAAA10420.10443623476303397No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGA10360.10383487448608754No Hit
GTGTACTTCTGTGCGAGAGGTCGGACTGGTATTACCCAAATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT10340.1036344210604387No Hit
TCCTGAGACTGGAGTCCACTGAAGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGTGAATCGGTCAGGGACC10300.10323351420914108No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10200.102231247080897No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10170.10193056694242376No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCT10150.10173011351677494No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10030.10052739296288205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3550.061.2410170
TGTCGTA252.5848678E-455.1225938
GGTATCA7950.043.0224721
TAGTCAC602.9422517E-740.19355438
AATAGTC602.989218E-740.11251436
CGTATGC3550.039.90608641
CTCGACG3800.039.63710828
ACTCGAC3800.039.62318827
GACTCGA3900.039.47474726
TCGTATG3600.039.3100240
TATACAC3750.039.20163
TCGACGT3900.038.63046329
TTATACA3850.038.1833762
GACGTCA3950.038.17404631
ACGAGAC3950.038.0860322
TGGTATC2450.037.6759532
AGACTCG4000.037.61938525
TACACAT4100.037.524685
TCTCCGA3850.037.2953911
CACGAGA3950.037.2167121
Produced by FastQC (version 0.11.5)