FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997738
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTGAGACTGGAGTCCACTGAAGGCCAGGGAGG55530.5565589363139422No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGG50910.5102541949890652No Hit
GTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCC46080.4618446926948758No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39590.3967975560718344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35800.35881163191138354No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCT35450.3553036969625292No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATG34840.3491898674802403No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32480.32553636325367985No Hit
GTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC28730.2879513459445265No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCACTGGCTCCAAG27900.2796325287801006No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27500.27562346026712425No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC25410.2546760772868228No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGG24750.24806111424041183No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24510.24565567313262598No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCAC23920.2397422970759859No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22930.22981985250636938No Hit
GATTATTACTTTGGATGAGGAGTTTGGGGCCCGTTCCCGGGAGTTGCTGG22880.22931871894224737No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22840.22891781209094975No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22360.22410692987537809No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22260.223104662747134No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20240.20285886675660345No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19300.19343755575110902No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19230.19273596876133814No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19220.19263574204851375No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATCCTGTAAATTGGTACCAGCAACTCCCGGGAACGGGCCC18730.18772463312011772No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18530.18572009886362953No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18300.18341488446866813No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18060.18100944336088232No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17970.18010740294546265No Hit
CCCCTGAGGGCCGCTGATTATTACTTTGGATGAGGAGTTTGGGGCCCGTTCCCGGGAGTTGCTGGTACCAATTTA17690.1773010549863792No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17590.1762987878581351No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17540.17579765429401306No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17440.17479538716576895No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17100.17138767892973908No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAAT16550.16587520972439657No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16010.1604629672318785No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15930.15966115352928323No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15880.1591600199651612No Hit
CCTCAGGGGTCCCTGACCGATTCACTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCTTCAGTGGACTCCAGT15460.15495049802653602No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14890.14923757539554472No Hit
AGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG14790.14823530826730064No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14520.1455291870210416No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14400.1443264664671487No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14340.14372510619020223No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14100.14131966508241645No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14060.1409187582311188No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT13940.1397160376772259No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG13480.13510560888730308No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC13460.13490515546165427No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13340.13370243490776137No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13150.1317981273640976No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTG13050.1307958602358535No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13020.1304951800973803No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12910.12939268625631178No Hit
CCTTGGTCCCTCCGCCGAACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATC12640.12668656501005274No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12570.12598497802028188No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC12500.12528339103051103No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12400.12428112390226692No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATCCT12380.12408067047661811No Hit
ACACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC12270.12297817663554961No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACAC12260.12287794992272522No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACACCATTCAGGC12220.12247704307142757No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC11990.12017182867646618No Hit
GAAGTACACAGCCGTGTCCTCGACTTTCAGGCTGTTCATTTGCAGATACA11780.11806706770715357No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11780.11806706770715357No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTG11650.11676412044043627No Hit
GTCTGGCACCTCAGCCTCCCTGGCCTTCAGTGGACTCCAGTCTCAGGATG11580.11606253345066542No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTGAGACTGGAGTCCAC11580.11606253345066542No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11580.11606253345066542No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA11530.11556139988654335No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11480.1150602663224213No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11280.11305573206593314No Hit
GTCCCTGACCGATTCACTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11240.1126548252146355No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11170.11195323822486465No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11050.11075051767097173No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG10960.10984847725555207No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10930.10954779711707884No Hit
GTTTGGGGCCCGTTCCCGGGAGTTGCTGGTACCAATTTACAGGATTATTT10890.1091468902657812No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT10770.1079441697118883No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10740.10764348957341507No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTACTTTGGATGAGGAGTTTGGGGCCCGTTCCCGGGAGTTGCTGGTA10700.10724258272211742No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAG10450.1047369149015072No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10410.10433600805020958No Hit
CCCTAAGACTCTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGCTTTGGC10350.10373464777326312No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10340.1036344210604387No Hit
TCCTGAGACTGGAGTCCACTGAAGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGTGAATCGGTCAGGGACC10140.10162988680395055No Hit
GTCTCAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTGTTCGGCGGAGGGAC10110.10132920666547732No Hit
GTGCCAGACTTGGAGCCAGTGAATCGGTCAGGGACCCCTGAGGGCCGCTG9990.1001264861115844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGA200.00653763853.32156860
GTGGTAT2450.048.810261
TGGTATC2500.043.6945342
ATGCATG1554.9112714E-1141.6736770
GTATCAA20200.039.748951
GGTATCA7350.038.583351
ACGGGTT451.0649993E-437.93498616
TATCAAC22550.035.877232
ATCAACG22950.035.096143
TCAACGC23300.034.275994
GTATTAT501.9703261E-434.1671871
TGCACCG2100.034.132936
AACGCAG23750.033.9173556
GCCAGTG23350.033.74942870
CAACGCA23800.033.7043725
ACGCAGA24750.032.545337
GTACGGA851.3154568E-732.157351
CGCAGAG25500.031.721968
CGAGAAA450.00391991531.71717862
AGAGTAC25700.031.60790311
Produced by FastQC (version 0.11.5)