FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251492
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14210.5650279134127527No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8480.33718766402112194No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA8270.3288374978130517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7170.28509853196125523No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6040.2401666852225916No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5750.22863550331620888No Hit
GTCTCTGGTGTCTCCATCACCGGTTACTCCTGGACTTGGATCCGGCAGCC5480.2178995753344043No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCGGTTACTCCTGGACTTGGATCCGGCAGCCCCCAGGGATGGGA5030.20000636203139663No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4950.19682534633308416No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCACCTATTTAAATTGGTATCAGCACAAA4890.19443958455934982No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4690.1864870453135686No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCAC4610.18330602961525616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4330.1721724746711625No Hit
GATCTATGGTGCGTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA4310.1713772207465844No Hit
GTAGTAGATTGCAAAATCGTCAGGTTGCAGACTGCCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA4280.1701843398597172No Hit
ATCTACTACTGTCAACAGATGTACACGTCTTTGTTCACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAA4270.16978671289742814No Hit
GTCTATTACTGTGCGAGACGAACACATATAGGCTTTAATTACTTCTTTGA4260.16938908593513907No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG4250.16899145897285003No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4220.16779857808598286No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4220.16779857808598286No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC4180.16620807023682663No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4170.16581044327453756No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4060.16143654668935792No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4020.15984603884020168No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3990.15865315795333448No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3860.15348400744357674No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3690.14672434908466273No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3660.14553146819779555No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC3600.14314570642406121No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3570.14195282553719402No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3550.1411575716126159No Hit
CTACTACTGTCAACAGATGTACACGTCTTTGTTCACTTTTGGCCAGGGGA3410.13559079414056907No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG3410.13559079414056907No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3400.13519316717828No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC3390.13479554021599097No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3380.1343979132537019No Hit
CATATAGGCTTTAATTACTTCTTTGACTCCTGGGGCCAGGGAGCCCTGGT3290.13081927059310036No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC3290.13081927059310036No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC3280.1304216436308113No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA3270.13002401666852226No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3260.1296263897062332No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT3210.12763825489478792No Hit
CCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTGTGCTGATACCAATT3160.12565012008334261No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3130.12445723919647543No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT3060.12167385046045202No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3050.12127622349816297No Hit
GATTTCACTCTCACCATCGGCAGTCTGCAACCTGACGATTTTGCAATCTA3040.1208785965358739No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3030.12048096957358485No Hit
GTCTGCAACCTGACGATTTTGCAATCTACTACTGTCAACAGATGTACACGTCTTTGTTCACTTTTGGCCAGGGGA3020.1200833426112958No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC2990.1188904617244286No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2870.1141189381769599No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2850.11332368425238178No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2820.1121308033655146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2760.10974504159178027No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA2750.1093474146294912No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC2720.10815453374262401No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT2690.10696165285575684No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG2660.10576877196888966No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT2660.10576877196888966No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA2640.10497351804431156No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC2640.10497351804431156No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC2630.10457589108202249No Hit
GGACTGGAGTGGATTGGCTATATCATTCACAGTGGGAGCACCAACTACAA2610.10378063715744439No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2600.10338301019515532No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC2560.10179250234599907No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC2540.10099724842142095No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2530.1005996214591319No Hit
AGTCAGACCATTGGCACCTATTTAAATTGGTATCAGCACAAACCAGGGAA2530.1005996214591319No Hit
CTCCTGGACTTGGATCCGGCAGCCCCCAGGGATGGGACTGGAGTGGATTGGCTATATCATTCACAGTGGGAGCAC2520.10020199449684285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTC150.001593641476.1221267
GTTCTAT150.002629637367.095311
TAACACG150.002633789367.068614
AGTTTGA200.00628340153.84623354
GCTTGTG200.00822562150.30146421
GTCGTAT552.6302587E-950.08201639
CACGGAT2550.049.9329770
TGTCGTA552.7121132E-949.9087638
ACGAACC305.3686684E-447.51933752
TTAATAG403.525324E-545.73852560
CGTATGC2450.045.27672241
TCGTATG2350.044.10808640
AAAGGGG1900.043.86981668
GTATGCC2550.043.59976242
TCTTATA851.8189894E-1243.397342
CTTATAC2400.043.3323861
TATACAC2400.043.3151443
AATCGTT405.0494178E-543.03923439
TAATCGT405.1537863E-542.8903438
TCAGATC551.8294122E-742.68002720
Produced by FastQC (version 0.11.5)