FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251492
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30671.2195218933405436No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7730.30736564184944254No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA7460.296629713867638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6330.2516978671289743No Hit
GTCTCTGGTGTCTCCATCACCGGTTACTCCTGGACTTGGATCCGGCAGCC5980.2377809234488572No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5750.22863550331620888No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5590.22227347191958394No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4910.19523483848392792No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCGGTTACTCCTGGACTTGGATCCGGCAGCCCCCAGGGATGGGA4560.18131789480381086No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4510.17932975999236556No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCACCTATTTAAATTGGTATCAGCACAAA4370.17376298252031874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4360.17336535555802968No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4320.17177484770887344No Hit
ATCTACTACTGTCAACAGATGTACACGTCTTTGTTCACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAA4320.17177484770887344No Hit
GATCTATGGTGCGTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA4170.16581044327453756No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCAC4170.16581044327453756No Hit
GTCTATTACTGTGCGAGACGAACACATATAGGCTTTAATTACTTCTTTGA4020.15984603884020168No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG3850.15308638048128767No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3750.1491101108583971No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3740.14871248389610803No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3690.14672434908466273No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3570.14195282553719402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3540.14075994465032685No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC3520.13996469072574874No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3500.1391694368011706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3500.1391694368011706No Hit
GTAGTAGATTGCAAAATCGTCAGGTTGCAGACTGCCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA3490.13877180983888154No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG3450.13718130198972533No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC3400.13519316717828No Hit
CCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTGTGCTGATACCAATT3400.13519316717828No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3380.1343979132537019No Hit
CTACTACTGTCAACAGATGTACACGTCTTTGTTCACTTTTGGCCAGGGGA3330.13240977844225663No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3330.13240977844225663No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT3310.1316145245176785No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3300.13121689755538946No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3250.12922876274394413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3210.12763825489478792No Hit
GATTTCACTCTCACCATCGGCAGTCTGCAACCTGACGATTTTGCAATCTA3200.12724062793249885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3200.12724062793249885No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3100.12326435830960826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3070.12207147742274108No Hit
CATATAGGCTTTAATTACTTCTTTGACTCCTGGGGCCAGGGAGCCCTGGT3060.12167385046045202No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC3060.12167385046045202No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA3040.1208785965358739No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG3010.11968571564900672No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC2970.11809520779985049No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2840.11292605729009272No Hit
GTCTGCAACCTGACGATTTTGCAATCTACTACTGTCAACAGATGTACACGTCTTTGTTCACTTTTGGCCAGGGGA2800.11133554944093649No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG2790.11093792247864744No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2730.10855216070491307No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC2710.10775690678033495No Hit
GGACTGGAGTGGATTGGCTATATCATTCACAGTGGGAGCACCAACTACAA2690.10696165285575684No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC2640.10497351804431156No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA2630.10457589108202249No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2600.10338301019515532No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2580.10258775627057719No Hit
GGCCAAAAGTGAACAAAGACGTGTACATCTGTTGACAGTAGTAGATTGCA2560.10179250234599907No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2550.10139487538371002No Hit
CTCCTGGACTTGGATCCGGCAGCCCCCAGGGATGGGACTGGAGTGGATTGGCTATATCATTCACAGTGGGAGCAC2530.1005996214591319No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2520.10020199449684285No Hit
AGTCAGACCATTGGCACCTATTTAAATTGGTATCAGCACAAACCAGGGAA2520.10020199449684285No Hit
CTATTACTGTGCGAGACGAACACATATAGGCTTTAATTACTTCTTTGACTCCTGGGGCCAGGGAGCCCTGGTCAC2520.10020199449684285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTT100.008304299137.4877370
TATTCTA100.008304299137.4877370
TTCAATG300.0023854268.74386670
AGAGGTT300.0023854268.74386670
GTAGTTT350.00439372658.92331370
AATGGTA609.279789E-657.28655270
ACAGTCT200.00834899650.112358
CACGGAT2050.040.2403170
GTATCAA5650.036.6895451
ACAGGGT400.003023331533.4348261
TATCAAC6700.030.9150812
TGGATTG3200.030.0754470
ATCAACG6900.030.0189933
TCAACGC7100.029.1733844
GGTCAAA950.00611052528.94478470
TAGGCAA950.00611052528.94478470
CGGATTA655.5707287E-428.60523664
CAACGCA7250.028.5697985
AACGCAG7300.028.3741136
TGATACT500.006768231728.35611749
Produced by FastQC (version 0.11.5)