FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051711
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT62910.5981681279362867No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42900.4079067348349499No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40490.3849916944864131No Hit
GTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT38960.3704439717755163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36560.34762401458195263No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32610.31006616836754586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31060.2953282793467027No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28260.26870499595421177No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27640.2628098403458745No Hit
ATTTTATACAACTCCGACAGTAAGAATTACTTAGCTTGGTTCCAGCAGAA26350.250544113354334No Hit
GGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT25740.24474404090096993No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24230.2303864845000195No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24100.22915040348536814No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23700.22534707728644082No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTA23330.22182900055243313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22860.2173600922686936No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22280.21184526928024905No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22100.21013377249073178No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21660.2059501136719118No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20890.19862871073897678No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18420.17514317146060085No Hit
CTCCTAAGTTGCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG18420.17514317146060085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17800.1692480158522636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17770.16896276638734406No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17560.16696602013290723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17320.16468402441355087No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16560.15745770463558906No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16530.15717245517066952No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA15800.15023138485762724No Hit
GTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAA15250.14500181133410223No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15000.1426247324597727No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATA14840.1411034019802018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14360.13653941054148905No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA14350.13644432738651588No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCAC14250.13549349583678405No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTAACTGGGCATCTACCCGGCAA14250.13549349583678405No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14090.13397216535721315No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTG14000.13311641696245452No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13970.13283116749753496No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13500.12836225921379543No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13490.12826717605882224No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAACTCCGA13030.12389335093005588No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12990.12351301831016316No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12960.12322776884524361No Hit
GAATTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGTTGC12490.11875886056150406No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12340.11733261323690633No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12230.11628669853220135No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12180.11581128275733542No Hit
TCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACC12100.11505061751754997No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11980.1139096196578718No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11970.1138145365028986No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11880.11295878810813997No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11760.1118177902484618No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11720.11143745762856906No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTACTGTCGGAGTTG11560.10991612714899815No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC11530.10963087768407861No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGAA11340.10782429773958815No Hit
ATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGA11180.10630296726001724No Hit
ATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCACCATCGGCAGCC11180.10630296726001724No Hit
GAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG10990.1044963873155268No Hit
GTATAAAATACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10960.10421113785060725No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10770.10240455790611679No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10750.10221439159617043No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10710.1018340589762777No Hit
TTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGA10690.10164389266633135No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10640.10116847689146542No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10560.10040781165167997No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10540.10021764534173361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.041.6463131
GTATCAA28000.036.9209751
TATCAAC32600.031.4816722
TGGTATC3350.031.4474412
TCAACGC32900.031.296414
ATCAACG33050.031.1543673
AACGCAG33400.030.9325876
CAACGCA33400.030.8293695
GCTTATA450.004960235730.22771
TGTTAGA14450.029.86387370
ACGCAGA35000.029.421427
CGCAGAG35300.029.1713818
AGAGTAC35700.029.03492411
CAGAGTA36200.028.54000710
GCATATC605.6919677E-428.51209831
AATGGTA3001.8189894E-1228.33297270
GCAGAGT36350.028.3287419
TCTGTCG16200.027.84864670
GTGGTAT3550.027.779681
TTAACGG1251.3281715E-724.71371335
Produced by FastQC (version 0.11.5)